Vpliv termina na količino in čistost izolirane dna iz listov sliv (prunus domestica l.) in bezgov (sambucus spp.)

Fekonja, Sara

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Title:	Vpliv termina na količino in čistost izolirane dna iz listov sliv (prunus domestica l.) in bezgov (sambucus spp.)
Authors:	ID Fekonja, Sara (Author) ID Šiško, Metka (Mentor) More about this mentor...
Files:	MAG_Fekonja_Sara_2019.pdf (3,43 MB) MD5: DCDFAB41E825489B4F68BC265DD29E20 PID: 20.500.12556/dkum/2b299b65-2d76-4751-9a43-d3e8dc64797c
Language:	Slovenian
Work type:	Master's thesis/paper
Organization:	FKBV - Faculty of Agriculture and Life Sciences
Abstract:	V naši raziskavi, ki je potekala v letu 2018 na Fakulteti za kmetijstvo in biosistemske vede Maribor (laboratorij za genetiko), smo primerjali dve različni metodi izolacije DNA: klasično metodo CTAB (cetil trimetil amonijev bromid) in metodo CTAB z dodanim PVP (polivinilpirolidon), proteinazo in RNazo, ki smo jo poimenovali metoda PVP. Oba protokola smo uporabili pri dveh rastlinskih vrstah: pri slivah (Prunus domestica L.) in pri bezgu (Sambucus spp.). Izolacijo DNA smo izvajali v pomladnih in jesenskih mesecih, saj nas je zanimala razlika v količini in čistosti izolirane DNA v povezavi s starostjo oziroma zrelostjo rastlinskih tkiv (listov). Ugotovili smo, da je koncentracija izolirane DNA bezga in sliv višja pri rastlinskem materialu, ki je bil nabran v pomladanskem obdobju, kot pa pri listih, ki so bili nabrani v septembru. Ugotovili smo tudi, da izbira genotipa vpliva na koncentracijo izolirane DNA. Rezultati namreč kažejo, da smo večjo količino DNA izolirali pri bezgu, saj je bila povprečna izmerjena koncentracija DNA pri bezgu 496,4 ng/μl, pri slivah pa 69,34 ng/μl.
Keywords:	Sambucus ssp. / Prunus domestica L. / izolacija DNA / CTAB / PVP
Place of publishing:	Maribor
Year of publishing:	2019
PID:	20.500.12556/DKUM-73095
NUK URN:	URN:SI:UM:DK:6OBOPAKJ
Publication date in DKUM:	22.02.2019
Views:	1692
Downloads:	196
Metadata:
Categories:	FKBV
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Licences

License:	CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

Link:	http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:	The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:	07.02.2019

Secondary language

Language:	English
Title:	Timing impact on the quantity and purity of isolated dna from prune (p. domestica l.) and elder (sambucus spp.) leaves
Abstract:	In our study, which took place in 2018 at the Faculty of Agriculture and Life Sciences Maribor (Genetics Laboratory), we compared two different methods of DNA isolation: the classic CTAB method (cetyl trimethyl ammonium bromide) and the CTAB method with added PVP (polyvinylpyrrolidone), proteinase, and RNase, which we named the PVP method. Both protocols were used in two plant species: in plums (Prunus domestica L.) and in elder (Sambucus spp.). The isolation of DNA was carried out in the spring and autumn months as we were interested in the difference in the amount and purity of isolated DNA in relation to the age or maturity of plant tissues (leaves). We found that the concentration of isolated elder and plum DNA was higher in the plant material collected during spring period than in the leaves that were collected in September. We also found that the genetic structure (genotype) affected the concentration of isolated DNA. The results indicate that a greater amount of DNA was isolated in elder; the mean measured DNA concentration of elder was 496.4 ng/μl and 69.34 ng/μl for plums.
Keywords:	Sambucus ssp. / Prunus domestica L. / DNA isolation / CTAB / PVP

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