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Title:Vzpostavitev tkivne kulture pri arabskem kavovcu (Coffea arabica L.)
Authors:ID Dobnikar, Nik (Author)
ID Šiško, Metka (Mentor) More about this mentor... New window
Files:.pdf UN_Dobnikar_Nik_2019.pdf (721,87 KB)
MD5: 3AA86FD32684EEF32E4F15D17A9E2591
PID: 20.500.12556/dkum/aaa74b8c-99f8-4411-87c8-bda7dd50d0e2
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKBV - Faculty of Agriculture and Life Sciences
Abstract:Kava je eden najpomembnejših kmetijskih pridelkov na mednarodnem trgu. Razmnoževanje in žlahtnjenje kavovca, na katerem zraste, traja več let, zato v sodobnem kmetijstvu uporabljamo tkivne kulture, s katerimi matično rastlino namnožimo, pospešimo prehod v generativno fazo in pridobimo zdrav rastlinski material. V diplomski nalogi smo vzpostavili tkivno kulturo pri arabskem kavovcu (Coffea arabica L.) iz aksilarnih meristemov. Za sterilizacijo smo uporabili tri načine: raztopino DICA, 1 % raztopino NaClO in 5 % raztopino NaClO; pri vseh treh smo izsečke obravnavali 5 min. Pri vsakem obravnavanju smo sterilizirali po 35 nodijev matične rastline. Najboljša je bila sterilizacija z 1 % NaClO, kjer smo dobili 19 (54,29 %) vitalnih brstov; okužen je bil en nodij; 15 jih je propadlo. Pri sterilizaciji z DICA smo dobili 14 (40,00 %) vitalnih brstov; okužili so se 4 nodiji; 17 jih je propadlo. Pri sterilizaciji s 5 % NaClO je bil okužen 1 nodij (2,86 %); vitalnih je ostalo 8 (22,86 %) rastlinic; kar 74,29 % jih je propadlo, kar kaže na previsoko koncentracijo NaClO. V drugem delu diplomske naloge smo spremljali razraščanje rastlin kavovca ob dodatku 6 mg/L rastlinskega hormona BA v gojišče. Po 44 dneh nobena od rastlin ni odgnala dodatnega poganjka. Povečalo se je samo število listov, ki se je pri 18 opazovanih rastlinicah povečalo iz povprečno 2,0 lista na rastlino na 3,17 listov na rastlino.
Keywords:kavovec, Coffea arabica L., tkivne kulture, sterilizacija, razraščanje
Place of publishing:Maribor
Year of publishing:2019
PID:20.500.12556/DKUM-73599 New window
NUK URN:URN:SI:UM:DK:JJFRI7MH
Publication date in DKUM:31.05.2019
Views:1375
Downloads:124
Metadata:XML DC-XML DC-RDF
Categories:FKBV
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:22.05.2019

Secondary language

Language:English
Title:Tissue culture establishment in arabian coffee (Coffea arabica L.)
Abstract:Coffee is one of the most important agricultural products on the international market. In modern agriculture, tissue cultures are used to multiply the mother plant, accelerate the transition to the generative phase and obtain healthy plant material. In the thesis, a tissue culture of Arabian coffee (Coffea arabica L.) was established. The sterilization of the mother plant material was carried out in three different treatments: with a DICA solution, with 1% NaClO solution and with 5% NaClO solution, all of which were treated for 5 min. In each treatment, 35 cuttings of the parent plant were sterilized. Sterilization with 1% NaClO was the most effective. Through this method we have obtained 19 (54,29%) of vital plants and 1 cutting (2,86%) was infected. 4 (11,43%) plants were infected under DICA sterilization, while 14(40,00%) remained vital. Sterilization with 5% NaClO resulted in 8 (22,86%) vital plants and 1 (2,86%) infected cutting, 74.29% of the cuttings died due to a too high concentration. In the second part of the thesis, plants with the addition of 6 mg / L of plant hormone BA to the medium were grown. After 44 days in the hormone infused media, none of the 18 observed plants grew any extra shoots. The number of leaves was the only factor that changed. From an average of 2.0 leaves per plant it changed to 3.17 leaves per plant.
Keywords:coffee, Coffea arabica L., tissue culture, sterilizatioN, multiplication


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