Primerjava koncentracij in čistosti dna izolirane iz brstov (generativnih in vegetativnih) in listov (svežih, posušenih in liofiliziranih) bezga  (sambucus nigra l.)

Jelen, Špela

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Title:	Primerjava koncentracij in čistosti dna izolirane iz brstov (generativnih in vegetativnih) in listov (svežih, posušenih in liofiliziranih) bezga (sambucus nigra l.)
Authors:	ID Jelen, Špela (Author) ID Šiško, Metka (Mentor) More about this mentor...
Files:	UN_Jelen_Spela_2019.pdf (1,68 MB) MD5: 3CD6CE3A18031042F74CC04104370E61 PID: 20.500.12556/dkum/1ad7827d-47e3-4ec2-a038-10ae3c922091
Language:	Slovenian
Work type:	Bachelor thesis/paper
Typology:	2.11 - Undergraduate Thesis
Organization:	FKBV - Faculty of Agriculture and Life Sciences
Abstract:	V letu 2019 smo na Fakulteti za kmetijstvo in biosistemske vede (laboratorij za genetiko) najprej izolirali DNA s CTAB metodo iz vegetativnih in generativnih brstov ter iz svežih, posušenih in liofiliziranih listov črnega bezga (Sambucus nigra). Primerjali smo koncentracije in čistost DNA. Namen diplomske naloge je bil ugotoviti iz katerih vzorcev smo dobili največjo koncentracijo DNA in iz katerih vzorcev je bila DNA najbolj čista. Koncentracije DNA smo izmerili s fluorimetrom, čistost pa s spektofotometrom in kvalitativno z agarozno elektroforezo. Ugotovili smo, da so bile največje koncentracije DNA pridobljene iz posušenih listov s povprečno vrednostjo 706,07 ng/µL. Najmanjše koncentracije DNA pa so bile izolirane iz svežih listov, s povprečno vrednostjo 301,13 ng/µL. Med vegetativnimi in generativnimi brsti ni bilo statistično značilnih razlik v koncentraciji in čistosti DNA. Prav tako ni bilo statistično značilnih razlik v čistosti med svežimi, posušenimi in liofiliziranimi listi. Najbližje optimalnemu razmerju A260/A280 so bili vzorci iz posušenih listov, s povprečnim razmerjem 2,08. Zaključili bi lahko, da dobimo največje koncentracije DNA iz posušenih listov, ki so se v našem primeru sušili dva tedna na zraku
Keywords:	Sambucus nigra L., izolacija DNA, brst, svež, posušen, liofiliziran list, koncentracije DNA
Place of publishing:	Maribor
Year of publishing:	2019
PID:	20.500.12556/DKUM-74107
NUK URN:	URN:SI:UM:DK:DDUROPTZ
Publication date in DKUM:	22.08.2019
Views:	1553
Downloads:	149
Metadata:
Categories:	FKBV
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Licences

License:	CC BY-ND 4.0, Creative Commons Attribution-NoDerivatives 4.0 International

Link:	http://creativecommons.org/licenses/by-nd/4.0/
Description:	Under the NoDerivatives Creative Commons license one can take a work released under this license and re-distribute it, but it cannot be shared with others in adapted form, and credit must be provided to the author.
Licensing start date:	06.08.2019

Secondary language

Language:	English
Title:	Comparison of Concentrations and DNA Purity in Generative and Vegetative Buds, and Fresh, Dried and Lyophilised Leaves of Black Elderberry (Sambucus nigra L.)
Abstract:	In 2019, in the genetics laboratory of the Faculty of Agricuture and Life Sciences, we began with isolation of DNA using the CTAB method from vegetative and generative buds, and from fresh, dried and lyophilized leaves of the black elderberry (Sambucus nigra). We compared DNA concentrations and purity levels. The purpose of the presented research was to find out which samples resulted in the highest concentrations of DNA, and which samples had the purest DNA. DNA concentrations were measured by the fluorimeter, whereas the DNA purity was determined by the spectrophotometer, and qualitatively evaluated by the agarose electrophoresis. The highest concentrations of DNA were obtained from dried leaves, having an average value of 706.07 ng / µL. The lowest DNA concentrations were obtained from fresh leaves, with an average value of 301.13 ng / µL. There were no statistically significant differences in DNA concentrations and purity levels between vegetative and generative buds. There were also no statistically significant differences in purity levels among fresh, dried or lyophilized leaves. The closest to the optimal A260/A280 value were the samples of dried leaves, with an average ratio of 2.08. Based on the results, it is possible to conclude that the highest concentrations of DNA were obtained from dried leaves (in our case, air-dried for two weeks).
Keywords:	Sambucus nigra L., DNA isolation, bud, fresh, dried, lyophilised leaf, DNA concentration

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