Vpliv načina sterilizacije in tipa brstov na uspešno vzpostavitev tkivne kulture pri breskvah (Prunus persica l.)

Guček, Marta

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Title:	Vpliv načina sterilizacije in tipa brstov na uspešno vzpostavitev tkivne kulture pri breskvah (Prunus persica l.)
Authors:	ID Guček, Marta (Author) ID Šiško, Metka (Mentor) More about this mentor...
Files:	VS_Gucek_Marta_2019.pdf (642,54 KB) MD5: B11EED831AF0D49C43F33B93B69DEE7F PID: 20.500.12556/dkum/08fd9cf3-4cca-485e-b299-5d4d7fd16a66
Language:	Slovenian
Work type:	Bachelor thesis/paper
Typology:	2.11 - Undergraduate Thesis
Organization:	FKBV - Faculty of Agriculture and Life Sciences
Abstract:	V letu 2018 in 2019 smo na Fakulteti za kmetijstvo in biosistemske vede v Hočah opravili poskus sterilizacije brstov in iniciacije tkivne kulture pri breskvah. V poskusu leta 2018 smo od 243 uspešno sterilizirali 5 brstov (tretiranje z dikloroizocianurno kislino (DICA 7 minut). Leta 2019 smo poskus ponovili, vključili smo 339 brstov (zaprtih in odprtih) vinogradske breskve iz genske banke koščičarjev na Fakulteti za kmetijstvo in biosistemske vede. Za sterilizacijo smo uporabili razkuževalni sredstvi DICA (tretiranje 3, 5 in 6 minut) in 5 % natrijevega hipoklorita (tretiranje 5 in 7 minut). Primerjava sterilizacije zaprtih in odprtih brstov je pokazala, da je bila uspešnejša sterilizacija pri odprtih brstih. Pri zaprtih brstih je prišlo do hitrega propadanja zaradi okužb. Z uporabljenimi načini sterilizacije smo uspešno vzpostavili sterilno kulturo. Najuspešnejša je bila sterilizacija z DICA 6 minut (2019) pri odprtih brstih, kjer smo dobili 2 sterilna in vitalna brsta.
Keywords:	breskev / in vitro / brsti / sterilizacija / tkivne kulture
Place of publishing:	Maribor
Year of publishing:	2019
PID:	20.500.12556/DKUM-74145
NUK URN:	URN:SI:UM:DK:ESNIG4OF
Publication date in DKUM:	22.08.2019
Views:	1528
Downloads:	194
Metadata:
Categories:	FKBV
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Licences

License:	CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

Link:	http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:	The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:	12.08.2019

Secondary language

Language:	English
Title:	The Effect of Sterilisation Technique and Type of Peach (Prunus persica L.) Bud on the Success of Tissue Culture Establishment
Abstract:	In 2018 and 2019, we carried out sterilization of peach buds and cultivar initiation at the Faculty of Agriculture and Life Sciences in Hoce. In the experiment carried out in 2018, we successfully sterilised 5 buds out of 243 (treatment for 7 minutes with dichloroisocyanuric acid (DICA)). We repeated the experiment in 2019 with 339 peach (open and closed) buds collected in the genebank of the Faculty. We used DICA desinfectant (treatments 3, 5 and 6 minutes) and 5 % sodium hypochlorite (treatments 5 and 7 minutes) for sterilisation. The comparison showed that the best sterilization was accomplished when treating open buds. Closed buds exhibited quick microbial infection, probably as a consequence of microorganisms hiding behind closed leaf enclosures. The applied sterilisation tehniques enabled us to establish a sterile culture. The most successful sterilisation was achieved with DICA when treating open buds for 6 minutes (2019) obtaining two sterile and viable sprouts.
Keywords:	Keywords: peach / in vitro / buds / sterilisation / tissue culture

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