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Title:Vpliv postopka ekstrakcije na kvaliteto ekstrakta iz Yerba mate (Ilex paraguariensis) : diplomsko delo univerzitetnega študijskega programa I. stopnje
Authors:ID Tratnik, Eva (Author)
ID Knez Marevci, Maša (Mentor) More about this mentor... New window
ID Kotnik, Petra (Comentor)
Files:.pdf UN_Tratnik_Eva_2020.pdf (1,66 MB)
MD5: 6BF4A9E92686DD48B99F800EDA0542AF
PID: 20.500.12556/dkum/7fbd326b-f01c-4fb3-952c-338612ed8378
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Namen diplomske naloge je ugotoviti, kako način in čas ekstrahiranja ter topilo vplivajo na končne ekstrakte, pridobljene iz posušenih listov južnoameriške rastline Yerba mate (Ilex paraguariensis). Izvedli smo ekstrakcijo po Soxhletu in superkritično ekstrakcijo s CO2 ob dodatku različnih topil in z različnimi časi ekstrahiranja. Ker je rastlina znana po zdravilnih in tudi poživljajočih učinkih, smo v ekstraktih analizirali dvoje; antioksidativno aktivnost ter vsebnost petih učinkovin; kofeina, teobromina, teofilina, kofeinske ter klorogenske kisline. Za določitev antioksidativnosti smo uporabili radikalsko metodo DPPH, za določitev učinkovin pa metodo tekočinske kromatografije z masno spektrometrijo. Želeli smo določiti optimalne pogoje ekstrakcije. Rezultati kažejo, da na kvaliteto ekstraktov vplivajo ekstrakcijska metoda, topilo ter čas ekstrakcije. Ugotovili smo, da je mešanica etanola in vode primernejše topilo kot etanol. Pri ekstrakcijah s tem topilom smo dobili višje izkoristke ter več ekstrahiranih aktivnih učinkovin, antioksidativna aktivnost pa je primerljiva. Najslabše rezultate kaže superkritična ekstrakcija s CO2, s kotopilom etanolom.
Keywords:Yerba mate, ekstrakcija po Soxhletu, superkritična ekstrakcija, LC-MS/MS, DPPH
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[E. Tratnik]
Year of publishing:2020
Number of pages:VIII, 26 str.
PID:20.500.12556/DKUM-77295 New window
UDC:665.7.038.5(043.2)
COBISS.SI-ID:30392835 New window
NUK URN:URN:SI:UM:DK:YZKBZSEH
Publication date in DKUM:24.09.2020
Views:1207
Downloads:155
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:25.08.2020

Secondary language

Language:English
Title:Influence of extraction procedure on the quality of Yerba mate (ilex paraguariensis) extract
Abstract:The purpose of this thesis is to determine how the method, time of extraction and solvent affect the final extracts obtained from the dried leaves of the South American plant Yerba mate (Ilex paraguariensis). Soxhlet extraction and supercritical extraction with different solvents and different extraction times were performed. Since the plant is known for its healing as well as invigorating effects, we analyzed two things; antioxidant activity and the content of five active ingredients; caffeine, theobromine, theophylline, caffeic and chlorogenic acids. The radical DPPH method was used to determine antioxidant properties, and the liquid chromatography method by mass spectrometry was used to determine the active ingredients. We wanted to determine the optimal extraction conditions. The results show that the quality of the extracts is influenced by the extraction method, solvent and extraction time. We found that a mixture of ethanol and water is a better solvent than ethanol. Extractions with this solvent gave higher yields and higher content of extracted active ingredients. The antioxidant activity was comparable. Supercritical extraction with CO2 and ethanol was performing the worst.
Keywords:yerba mate, Soxhlet extraction, supercritical extraction, LC-MS/MS, DPPH


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