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Title:Stabilnost lakaze v SC CO2 : diplomsko delo univerzitetnega študijskega programa I. stopnje
Authors:ID Švec, Mihaela (Author)
ID Primožič, Mateja (Mentor) More about this mentor... New window
ID Leitgeb, Maja (Comentor)
Files:.pdf UN_Svec_Mihaela_2020.pdf (1,93 MB)
MD5: 874F6641C21129A17EFE00D3DAF5EDCD
PID: 20.500.12556/dkum/13da7340-ec19-495e-a2a3-1178749e3af1
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Diplomsko delo zajema študijo stabilnosti encima lakaze v neraztopljeni obliki (praškasti obliki) in raztopljenega v pufru v superkritičnem ogljikovem dioksidu (SC CO2). Pri tem smo uporabljali šaržni reaktor, v katerem je bil encim izpostavljen SC CO2 pri različnih pogojih. Proučili smo kakšen vpliv ima izpostavitev obeh oblik lakaz v SC CO2 pri različnih temperaturah in tlakih. Ugotovili smo, da na stabilnost lakaze v SC CO2 vpliva tako tlak kot temperatura. Večji vpliv na preostalo aktivnost ima temperatura, saj smo pri višji temperaturi izpostavitve vzorcev v SC CO2 zasledili večji upad v preostali aktivnosti neglede na izbrani tlak. Izkazalo se je, da je lakaza v praškasti obliki bolj stabilna v SC CO2 kot lakaza raztopljena v pufru.
Keywords:Trametes versicolor, encim, lakaza, stabilnost lakaze, superkritični ogljikov dioksid, encimski aktivnostni test
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[M. Švec]
Year of publishing:2020
Number of pages:X, 34 str.
PID:20.500.12556/DKUM-77389 New window
UDC:577.151:637.345(043.2)
COBISS.SI-ID:31859459 New window
NUK URN:URN:SI:UM:DK:LZ4GZTTG
Publication date in DKUM:08.10.2020
Views:1217
Downloads:139
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:28.08.2020

Secondary language

Language:English
Title:Stability of laccase in SC CO 2
Abstract:The diploma presents a study of the stability of the enzyme laccase in undissolved form (powder form) and dissolved in buffer in supercritical carbon dioxide (SC CO2). The batch reactor in which the enzyme was exposed to SC CO2 under different conditions was used. The effect of exposure to both forms of laccase in SC CO2 at different temperatures and pressures was studied. We found that both pressure and temperature influence the stability of laccase in SC CO2. Temperature has a greater influence on the residual activity, since the activity of the samples in SC CO2 was lower at higher temperature, regardless of pressure. Laccase in powder form has been shown to be more stable in SC CO2 than laccase dissolved in buffer.
Keywords:Trametes versicolor, enyzme, laccase, stability of laccase, supercritical carbon dioxide, enyzme activity assay


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