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Title:Vpliv saharoze, sorbitola in temperature na in vitro rast in ohranjanje ozkolistnega koščca (Berula erecta )
Authors:ID Oblonšek, Sanja (Author)
ID Ambrožič-Dolinšek, Jana (Mentor) More about this mentor... New window
ID Ciringer, Terezija (Comentor)
Files:.pdf MAG_Oblonsek_Sanja_2021.pdf (1,70 MB)
MD5: 81D67C902BEB7EDB857872DF6512B642
PID: 20.500.12556/dkum/97f377c0-07e3-49bb-b5de-f9e38233b01b
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:V magistrskem delu smo raziskovali možnosti za hrambo ozkolistnega koščca (Berula erecta) v tkivni kulturi z upočasnjeno rastjo. Upočasnitev rasti smo poskušali doseči z nižanjem temperature in dodajanjem sladkorjev in sladkornih alkoholov na gojišče MS. Ugotavljali smo vpliv nizkih temperatur (13 °C in 4 °C), različnih kombinacij in koncentracij sorbitola (20, 40 g L-1) in saharoze (30, 40 g L-1) ter svetlobe na viabilnost, rast in barvila ozkolistnega koščca. Spremljali smo različne rastne parametre, vsebnost fotosinteznih barvil in antocianinov. Povišanje koncentracije saharoze ni zavrlo oziroma upočasnilo rasti. Pri 23 ± 2 °C je dodatek 20 g L-1 sorbitola uspešno zavrl rast, dodatek 40 g L-1 sorbitola pa je po dveh mesecih kulture vodil v senescenco rastlin. Hramba Berule erecta pri 13 °C v nobenem tretmaju ni bila učinkovita. Kot najbolj učinkovit način za dolgoročno hrambo B. erecta z minimalno rastjo in visoko viabilnostjo se je izkazala hramba rastlin pri 4 °C v temi.
Keywords:počasna rast, Berula erecta, rastni parametri, biokemijski parametri, saharoza, sorbitol, temperatura
Place of publishing:Maribor
Publisher:[S. Oblonšek]
Year of publishing:2021
PID:20.500.12556/DKUM-78481 New window
UDC:581.5:582.795(043.2)
COBISS.SI-ID:54272003 New window
NUK URN:URN:SI:UM:DK:9VV7WFNU
Publication date in DKUM:11.03.2021
Views:1496
Downloads:118
Metadata:XML DC-XML DC-RDF
Categories:FNM
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:12.01.2021

Secondary language

Language:English
Title:Effects of sucrose, sorbitol and temperature on in vitro growth and storage of Berula erecta
Abstract:In the master's thesis we investigated the possibilities for slow growth storage of Berula erecta. By lowering the temperature and adding sugars and sugar alcohols to the MS medium we attempted to reach growth retardation. We researched the influence of low temperatures (13 ° C and 4 ° C), various combinations of both, concentrations of sorbitol (20, 40 g L-1) and sucrose (30, 40 g L-1) and light on the viability, growth and photosynthetic pigments of B. erecta. We monitored various growth parameters, photosynthetic pigments and anthocyanins. The increase of sucrose concentration did not inhibit or decelerate growth. At 23 ± 2 ° C, the addition of 20 g L-1 sorbitol successfully inhibited growth, and the addition of 40 g L-1 sorbitol led to plant senescence after two months of in vitro storage. Storage of Berula erecta at 13 ° C was not effective in any treatment. Storing the plants at 4 ° C in the darkness proved to be the most effective long-term way to store B. erecta with minimal growth and high viability.
Keywords:slow growth, Berula erecta, growth parameters, biochemical parameters, sucrose, sorbitol, temperature


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