Bezgovi ekstrakti in inhibitorni potencial na mikrobe

Korpič, Špela

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Title:	Bezgovi ekstrakti in inhibitorni potencial na mikrobe
Authors:	ID Korpič, Špela (Author) ID Langerholc, Tomaž (Mentor) More about this mentor... ID Ivančič, Anton (Comentor)
Files:	MAG_Korpic_Spela_2021.pdf (3,53 MB) MD5: FBDF281E4DCBAB79F08F8099E03A5816 PID: 20.500.12556/dkum/7e8b4145-7df3-40dc-a7ed-53befe67fde7
Language:	Slovenian
Work type:	Master's thesis/paper
Typology:	2.09 - Master's Thesis
Organization:	FKBV - Faculty of Agriculture and Life Sciences
Abstract:	V magistrskem delu je bil preučevan učinek ekstraktov bezgovih jagod Sambucus nigra in jagod različnih medvrstnih križancev, ki so obsegali različne kombinacije S. javanica s S. nigra, S. cerulea in/ali S. racemosa, na dva pomembna prehranska patogena, to sta Salmonella typhimurium in Staphylococcus aureus. Ob prisotnosti ekstraktov bezgovih jagod v bakterijskem mediju se je spremljala rast koncentracije bakterij. Čeprav je bila rast testnih bakterij spremenjena ob prisotnosti bezgovih ekstraktov, minimalne inhibitorne koncentracije ni bilo mogoče določiti. Ugotovljeno je bilo, da se ob dodatku ekstraktov bezgovih jagod z višjo koncentracijo pospeši rast S. typhimurium in S. aureus, pri nizkih koncentracijah ekstraktov pa učinka na povečano rast koncentracije bakterij ni bilo zaznati. Največja hitrost rasti koncentracije bakterij ob dodatku večine ekstraktov bezgovih jagod je bila izmerjena pri koncentraciji ekstraktov 5 mg/mL. Tudi koncentracija bakterij po 24 urah je bila v tem primeru največja. Najvišja koncentracija bakterij S. typhimurium po 24 urah je bila dosežena ob prisotnosti ekstrakta bezgovih jagod JA × RAC, JA × KOR in JA × NI; pri istih bezgih je bila izmerjena tudi največja maksimalna hitrost rasti bakterij. Najmanjša maksimalna hitrost rasti je bila izmerjena ob dodatku ekstrakta bezga JA × (JA × NI). Pri bakteriji S. aureus je najvišjo koncentracijo po 24 urah povzročil dodatek ekstraktov bezgovih jagod S. nigra 'Black Beauty' in (JANI × CER) × MIQ. Največja maksimalna hitrost rasti bakterije za S. aureus je bil zaznana ob dodatku bezgovih jagod JA × KOR in križanca (JA × CER) × (JA × NI). Najmanjša maksimalna hitrost rasti je bila izmerjena ob dodatku ekstrakta bezga JA Addis Abeba.
Keywords:	Sambucus nigra, medvrstni križanci, inhibitorni potencial, mikrobi, bezgove jagode
Place of publishing:	Maribor
Year of publishing:	2021
PID:	20.500.12556/DKUM-78569
NUK URN:	URN:SI:UM:DK:JEK5M0YG
Publication date in DKUM:	17.02.2021
Views:	1262
Downloads:	164
Metadata:
Categories:	FKBV
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Licences

License:	CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

Link:	http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:	The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:	18.01.2021

Secondary language

Language:	English
Title:	Elderberry extracts and their potential to inhibit microbes
Abstract:	The thesis is associated with the investigation of the effect of berry extracts of Sambucus nigra and various interspecific combinations involving S. javanica with S. nigra, S. cerulea and/or S. racemosa against two important food pathogens Salmonella typhimurium and Staphylococcus aureus. Bacterial growth was monitored in media containing elderberry extracts. Although the growth of bacteria was altered in the presence of elderberry extracts, the minimum inhibitory concentration could not be determined. It was discovered that the addition of elderberry extracts in higher concentrations accelerated the growth of S. typhimurium and S. aureus, while at low concentrations the effect on the increased growth of the bacteria was not observed. The maximum bacterial growth rate in the presence of most elderberry extracts was measured in the presence of 5 mg/mL extract concentration. The concentration of bacteria reached after 24 hours, in the presence of 5 mg/mL extract, was also the highest in most cases. The highest concentration of S. typhimurium after 24 hours and the maximum growth rate of bacteria was reached in the presence of elderberry extracts JA × RAC, JA × KOR and JA × NI. The lowest maximum growth rate was measured with the addition of elderberry extract JA × (JA × NI). At S. aureus, the highest concentration after 24 hours was achieved with the addition of elderberry extracts S. nigra 'Black Beauty' and (JANI × CER) × MIQ. The highest maximum growth rate of S. aureus was detected with the addition of JA × KOR and hybrid (JA × CER) × (JA × NI). The lowest maximum growth rate was measured with the addition of elderberry extract JA Addis Abeba.
Keywords:	Sambucus nigra, interspecific hybrids, inhibitory effect, microbes, elderberry fruits

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