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Title:Razvoj kolorimetričnega senzorskega receptorja za zaznavanje biogenih aminov : diplomsko delo univerzitetnega študijskega programa I. stopnje
Authors:ID Bjelovučić, Gal (Author)
ID Finšgar, Matjaž (Mentor) More about this mentor... New window
ID Mastnak, Tinkara (Comentor)
Files:.pdf UN_Bjelovucic_Gal_2021.pdf (2,65 MB)
MD5: 8136E2FE96EC6233D397C3DCF17B6532
PID: 20.500.12556/dkum/379c51ea-20db-48a8-b82c-0b5ab95cc9d8
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Diplomsko delo predstavlja razvoj kolorimetričnega senzorskega receptorja (KSR) za zaznavanje trimetilamina, ki skupaj z dimetilaminom in amonijakom tvori enega glavnih parametrov kakovosti hrane ‒ celokupni hlapni bazični dušik ali TVB-N (angl. total volatile basic nitrogen). KSR, ki smo ga razvili, je sestavljen iz izključno z živili združljivih materialov: indikatorja (izvleček vijoličnega korenja) in polimerne osnove (derivat celuloze), ki smo ju v obliki polimerne raztopine nanesli na nosilec iz PET-folije . Za spremljanje barvnih sprememb tako pripravljenega KSR smo pripravili zaprt sistem, ki posnema pogoje v živilski embalaži ter vanj s tehniko vzorčenja iz nezapolnjenega prostora nad vzorcem injicirali analit. Kolorimetrično detekcijo in RGB-analizo smo izvedli s pametnim telefonom in brezplačno dostopno aplikacijo Color Grab. Potrdili smo, da so optične lastnosti indikatorja odvisne od koncentracije trimetilamina in v izbranem koncentracijskem območju dobili barvno lestvico od rdeče-vijolične do temno sive barve. Ugotovitve diplomske naloge so dobro izhodišče za nadaljnje raziskave in izpopolnjevanje tako KSR kot tudi metode. Ker kolorimetrična detekcija s pametnim telefonom ne zahteva usposobljenega osebja in je veliko cenejša ter hitrejša od uveljavljenih instrumentalnih metod, ki se sicer uporabljajo za določanje biogenih aminov, ima ta metoda velik potencial na področju spremljanja kakovosti in varnosti hrane. Morebitna uporaba kolorimetričnih senzorskih receptorjev v sklopu pametnega pakiranja živil bi lahko znatno zmanjšala količino zavržene hrane.
Keywords:kolorimetrični senzorski receptor, biogeni amini, trimetilamin, kontrola kakovosti hrane
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[G. Bjelovučić]
Year of publishing:2021
Number of pages:VIII, 33 str.
PID:20.500.12556/DKUM-79673 New window
UDC:577.354:547.233(043.2)
COBISS.SI-ID:80075011 New window
Publication date in DKUM:22.09.2021
Views:1326
Downloads:129
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:24.08.2021

Secondary language

Language:English
Title:Development of a colorimetric sensor receptor for the detection of biogenic amines
Abstract:The bachelor thesis presents the development of a colorimetric sensor receptor (CSR) for the detection of trimethylamine. Along with dimethylamine and ammonia, this analyte forms one of the main food quality parameters – total volatile basic nitrogen (TVB-N). The developed CSR consists of entirely food-compatible materials: an indicator (black carrot extract) and a polymer matrix (cellulose derivative), from which a polymer solution was prepared to later coat a solid-state substrate (a PET-foil). For the purpose of monitoring the color changes of CSR, a closed system that mimics conditions in food packaging was created and analyte injected according to the headspace method. Colorimetric detection and RGB analysis were performed using a smartphone and a commercially available application Color Grab. We confirmed that optical properties of the indicator depend on the concentration of trimethylamine and observed visible color changes from red-purple to dark gray. The presented findings are a good starting point for further improvement of the CSR and the method. Smartphone-based colorimetric detection does not require trained personnel and is much cheaper and faster than conventional instrumental techniques for the determination of biogenic amines. Therefore, sensing systems compatible with smartphone-based colorimetric detection offer a promising solution for monitoring of food quality and food safety. CSR integrated into smart food packaging have the potential to significantly reduce the amount of food waste.
Keywords:colorimetric sensor receptor, biogenic amines, trimethylamine, food quality control


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