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Title:Izolacija keratina iz odpadne biomase in sinteza keratinskih nanodelcev : diplomsko delo univerzitetnega študijskega programa I. stopnje
Authors:ID Mešl, Anja (Author)
ID Škerget, Mojca (Mentor) More about this mentor... New window
ID Fras Zemljič, Lidija (Comentor)
ID Plohl, Olivija (Comentor)
Files:.pdf UN_Mesl_Anja_2021.pdf (3,04 MB)
MD5: 962D64803B6038C4F84BAE220A3B5342
PID: 20.500.12556/dkum/579ca057-d448-4a22-b39b-08b805522edd
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Piščančje perje, ki je odpadna biomasa, predstavlja do 10 % celotne piščančje suhe mase. Iz tega vidika ima veliko potencialnih industrijskih aplikacij, pri tem pa tudi zmanjšamo količino odpadne biomase in vplivamo pozitivno na okoljski vidik. Skoraj v celoti je perje sestavljeno iz proteina keratina. Ker pa je keratin netopen v vodi, je njegova ekstrakcija zahtevna naloga. Večina dosedanjih procesov ekstrakcije je okolju neprijaznih in neekonomičnih. Raziskave torej vodijo v iskanje bolj zelenih in ekonomičnih procesov. V diplomski nalogi smo se odločili za okolju prijazen hidrotermični proces; ekstrakcijo keratina v subkritični vodi. Ekstrakcijo z vodo smo izvedli pri 180 °C in času 1h (2 g perja v 70 mL vode). Rezultati kažejo, da je naša suspenzija vsebovala keratin, katerega molekulske mase so bile v območju od 4,6 do 15 kDa. Z metodo ATR-FTIR smo potrdili prisotnost proteina keratina v ekstraktu. V nadaljevanju smo iz izoliranega keratina z alginatom, hitozanom in TPP z metodo kompleksacije in ionske gelacije sintetizirali delce, ki pa niso bili v nano območju tako, kot smo predvidevali. Sintetizirani delci so bili mikronskih velikosti in predstavljajo mikrodelce, ki smo jih analizirali. V njih smo nato ujeli zdravilo in določili kinetiko sproščanja. Ugotovili smo, da keratinske delce lahko uporabimo kot dostavni sistem za kontrolirano sproščanje učinkovin. Z nadaljnjo optimizacijo postopkov bi v prihodnosti lahko sintetizirali nanodelce.
Keywords:keratin, nanodelci, hidrotermični procesi, ekstrakcija v subkritični vodi, kompleksacija, perje
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[A. Mešl]
Year of publishing:2021
Number of pages:X, 44 str.
PID:20.500.12556/DKUM-80194 New window
UDC:66.061.3:547.962.9(043.2)
COBISS.SI-ID:79678467 New window
Publication date in DKUM:22.09.2021
Views:1043
Downloads:154
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:01.09.2021

Secondary language

Language:English
Title:Isolation of keratin from waste biomass and synthesis of keratin nanoparticles
Abstract:Chicken feathers, which are a waste product of biomass, account for up to 10% of total chicken dry matter. From this point of view, it has many potential industrial applications while reducing the amount of biomass waste and having a positive impact on the environmental aspect. Feathers are composed almost entirely of the protein keratin. However, since keratin is insoluble in water, its extraction is a difficult task. Most of the existing extraction methods are environmentally unfriendly and uneconomical. Therefore, research is on to find more environmentally friendly and economical methods. In the thesis, we have chosen an eco-friendly hydrothermal process; extraction of keratin in subcritical water. The extraction with water was carried out at 180 ° C and for 1 h (2 g of feathers in 70 mL of water). The results show that our suspension contained keratin whose molecular weights ranged from 4.6 to 15 kDa. The presence of keratin protein in the extract was confirmed by ATR-FTIR method. Subsequently, particles of isolated keratin were synthesized with alginate, chitosan and TPP by complexation and ionic gelation method, but they were not in the nanoscale as predicted. The synthesized particles were size of micrometer and represent the microparticles that we analyzed. We then entrapped the drug within them and determined the release kinetics. We found that keratin particles can be used as a delivery system for controlled drug release. By further optimizing the processes, nanoparticles could be synthesized in the future.
Keywords:keratin, nanoparticles, hydrothermal processes, extraction in subcritical water, complexation, feather


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