| Title: | Production of enzymes from medicinal mushrooms : master's thesis |
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| Authors: | ID Herranz Gómez, Gonzalo (Author)
ID Leitgeb, Maja (Mentor) More about this mentor... 
ID Primožič, Mateja (Comentor)
ID Kučuk, Nika (Comentor) |
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| Files: |  MAG_Herranz_Gómez_Gonzalo_2022.pdf (2,56 MB)
MD5: 33E4EA58A01E16E35D2298520654649B
PID: 20.500.12556/dkum/79253d24-98bf-41c3-9208-eddad1e3326b
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| Language: | English |
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| Work type: | Master's thesis/paper |
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| Typology: | 2.09 - Master's Thesis |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | The purpose of this master's thesis was to determine the ability of certain organisms of the kingdom Fungi to produce a series of enzymes in their active form by solid-state fermentation. In this study, two types of fungi were used, Pleurotus ostreatus and Ganoderma lucidum. For this purpose, different growth media, cultivation times (8 and 10 days), extraction procedures (shaking and homogenization) and extraction medium (distilled water, sodium citrate buffer and sodium phosphate buffer) were used.
First, for P. ostreatus mushroom, the optimization of the extraction procedure and time for isolation of enzymes in their active form (α-amylase, glucoamylase, cellulase, laccase, and protease) was studied. It was observed that the highest total protein concentration in mycelium extract was obtained by 8 min of homogenization (0.8607 mg/mL, and distilled water). Using the shaking procedure, the highest enzyme activities were achieved for α-amylase (24 h, 8.0413 U/mL, and sodium citrate buffer) and protease (3 h, 0.0040 U/mL, and sodium citrate buffer). With the homogenization process, the highest activities were achieved for the enzymes glucoamylase (10 min, 6.7113 U/mL, and sodium citrate buffer) and laccase (8 min, 12.2500 U/mL, and sodium citate buffer).
For the mushroom G. lucidum, the growth medium and the extraction procedure were optimized, using the same extraction medium (sodium citrate buffer). In this case, α-amylase, glucoamylase, cellulase, laccase, protease, catalase, peroxidase, superoxidase dismutase (SOD), and lipase were studied. It was observed that the highest total protein concentration was obtained with 4 min of homogenization (0.0338 mg/mL). Furthermore, using the homogenization process, the highest activities were achieved for α-amylase (4 min, 16.3459 U/mL) and SOD (4 min, 9.2615 U/mL). With the shaking procedure, the highest activities were achieved for cellulase (3 h, 1.6332 U/mL), lipase (3 h, 16.924 U/mL), glucoamylase (3 h, 14.6737 U/mL), peroxidase (3 h, 0.0156 U/mL), protease (3 h, 0.0080 U/mL) and laccase (24 h, 20.7083 U/mL). |
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| Keywords: | medicinal mushrooms, Pleurotus ostreatus, Ganoderma lucidum, total proteins, enzymes activities. |
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| Place of publishing: | Maribor |
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| Place of performance: | Maribor |
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| Publisher: | [G. Herranz Gomez] |
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| Year of publishing: | 2022 |
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| Number of pages: | 1 spletni vir (1 datoteka PDF (XIII, 53 f.)) |
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| PID: | 20.500.12556/DKUM-81955 |
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| UDC: | 604.4:577.15(043.2) |
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| COBISS.SI-ID: | 115283203 |
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| Publication date in DKUM: | 06.07.2022 |
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| Views: | 2069 |
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| Downloads: | 53 |
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| Metadata: |    |
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| Categories: | KTFMB - FKKT
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