Priprava umetnih semen plazeče zelene (Apium repens (Jacq.) Lag.) in vpliv temperature ter časa shranjevanja na regeneracijo : magistrsko delo

Pucko, Ines

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Title:	Priprava umetnih semen plazeče zelene (Apium repens (Jacq.) Lag.) in vpliv temperature ter časa shranjevanja na regeneracijo : magistrsko delo
Authors:	ID Pucko, Ines (Author) ID Ambrožič-Dolinšek, Jana (Mentor) More about this mentor... ID Ciringer, Terezija (Comentor)
Files:	MAG_Pucko_Ines_2023.pdf (711,98 KB) MD5: 9B5B9FAFA6CF3BE024CB8BCBA6F6532B
Language:	Slovenian
Work type:	Master's thesis/paper
Typology:	2.09 - Master's Thesis
Organization:	FNM - Faculty of Natural Sciences and Mathematics
Abstract:	Umetna semena se uporabljajo za daljše shranjevanje ogroženih, redkih, zdravilnih in industrijsko pomembnih rastlinskih vrst in lažji prenos v in vivo okolje. Plazeča zelena, Apium repens velja za ogroženo in redko rastlinsko vrsto, v Sloveniji nazadnje potrjeno pred več kot 100 leti. Cilj naše naloge je bil pripraviti umetna semena plazeče zelene (Apium repens), pridobljeno iz bližnjih rastišč, ki jo hranimo v tkivni kulturi na Fakulteti za naravoslovje in matematiko, Univerze v Mariboru. Umetna semena smo pripravili iz izsečkov, nodijev izoliranih z matične rastline A. repens. Izsečke smo takoj po izolaciji z matične rastline prenesli v MS gojišča z 2 µM BAP, 3-% natrijevim alginatom in 0,1 M in 0,4 M saharozo. Od tod smo nodije prenesli v MS gojišča s CaCl2, kjer so se zaradi polimerizacije oblikovali v kapsule. Kapsule umetnih semen smo hranili 2, 8 in 16 tednov pri temperaturah 23 °C, 15 °C in 4 °C ter spremljali živost umetnih semen. Živost in razvoj v poganjke in korenine inkapsuliranih in neinkapsuliranih nodijev smo ugotavljali na MS gojišču in mešanici perlita in MS gojišča, oboje z dodatkom 2 µM BAP. Umetna semena Apium repens inkapsulirana z 0,4 M saharozo, ki so bila takoj prenesena na gojišče, so preživela 50 % in ostala živa po 2 tednih pri temperaturi 15 °C (50 %) in temperaturi 4 °C (35 %) in po 8 tednih pri temperaturi 15 °C in 4 °C (5 %) ter po 16 tednih shranjevanja pri temperaturi 4 °C (8 %). Umetna semena Apium repens inkapsulirana z 0,1 M saharozo, ki so bila takoj prenesena na gojišče so preživela 92 %, po 2 tednih pri temperaturi 15 °C (43 %) in temperaturi 4 °C (35 %) ter po 8 tednih shranjevanja pri temperaturu 4 °C (37 %). Preživetje in rast rastlin se je s podaljševanjem časa shranjevanja umetnih semen zmanjševala, rast rastlin pa je bila upočasnjena.
Keywords:	Plazeča zelena, inkapsulacija, ogrožene rastlinske vrste, redke rastlinske vrste, tkivna kultura, in vitro
Place of publishing:	Maribor
Place of performance:	Maribor
Publisher:	[I. Pucko]
Year of publishing:	2023
Number of pages:	IX f., 40 str.
PID:	20.500.12556/DKUM-84174
UDC:	582.795(043.2)
COBISS.SI-ID:	152370947
Publication date in DKUM:	17.05.2023
Views:	907
Downloads:	101
Metadata:
Categories:	FNM
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Licences

License:	CC BY 4.0, Creative Commons Attribution 4.0 International

Link:	http://creativecommons.org/licenses/by/4.0/
Description:	This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:	24.04.2023

Secondary language

Language:	English
Title:	Preparation of artificial seed apium repens (Jacq.) Lag. and the influence of temperature and storage time on regeneration
Abstract:	Artificial seeds are used to preserve endangered, rare, medicinal, and industrially important plant species longer and to facilitate their transfer to the in vivo environment. The creeping marshwort, Apium repens, is considered an endangered and rare plant species, last confirmed in Slovenia more than 100 years ago. The aim of our work was to produce artificial seeds of creeping bentgrass (Apium repens) obtained from nearby sites and stored in tissue culture at the Faculty of Natural Sciences and Mathematics, University of Maribor. Artificial seeds were produced from cuttings isolated from the parent plant of A. repens. Immediately after isolation from the mother plant, the cuttings were transferred to MS media containing 2 µM BAP, 3% sodium alginate and 0.1 M and 0.4 M sucrose. From there, the nodules were transferred to MS media containing CaCl2 where they formed capsules by polymerization. The artificial seed capsules were stored at 23 °C, 15 °C, and 4 °C for 2, 8, and 16 weeks, respectively, and the viability of the artificial seeds was monitored. Viability and development into shoots and roots of encapsulated and non-encapsulated nodules were determined on MS medium and a mixture of perlite and MS medium, both supplemented with 2 µM BAP. Artificial Apium repens seeds encapsulated with 0.4 M sucrose and immediately transferred to medium survived 50% and remained alive after 2 weeks at 15 °C (50%) and 4 °C (35%) and after 8 weeks at 15 °C and 4 °C (5%) and after 16 weeks of storage at 4 °C (8%), after 2 weeks at 15 °C (50%) and 4 °C (35%) and after 8 weeks at 15 °C and 4 °C (5%) and after 16 weeks of storage at 4 °C (8%), 4% of the seeds survived and remained alive. Artificial seeds of Apium repens encapsulated with 0.1 M sucrose and immediately transferred to the medium survived 92%, after 2 weeks at 15 °C (43%) and 4 °C (35%), and after 8 weeks of storage at 4 °C (37%). The survival rate and growth of the plants decreased with increasing storage time of the artificial seeds, and plant growth was slowed down.
Keywords:	Creeping celery, encapsulation, endangered species, rare species, tissue culture, in vitro

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