| Title: | The effect of the Ala16Val mutation on the secondary structure of the manganese superoxide dismutase mitochondrial targeting sequence |
|---|
| Authors: | ID Broz, Matic (Author)
ID Furlan, Veronika (Author)
ID Lešnik, Samo (Author)
ID Jukič, Marko (Author)
ID Bren, Urban (Author) |
|---|
| Files: |  The_Effect_of_the_Ala16Val_Mutation-Broz-2022.pdf (5,14 MB)
MD5: D846FA3D80E007D814B11A356A282C61
 https://www.mdpi.com/2076-3921/11/12/2348
|
|---|
| Language: | English |
|---|
| Work type: | Scientific work |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
|
|---|
| Abstract: | Manganese Superoxide Dismutase (MnSOD) represents a mitochondrial protein that scavenges reactive oxygen species (ROS) responsible for oxidative stress. A known single nucleotide polymorphism (SNP) rs4880 on the SOD2 gene, causing a mutation from alanine to valine (Ala16Val) in the primary structure of immature MnSOD, has been associated with several types of cancer and other autoimmune diseases. However, no conclusive correlation has been established yet. This study aims to determine the effect of the alanine to valine mutation on the secondary structure of the MnSOD mitochondrial targeting sequence (MTS). A model for each variant of the MTS was prepared and extensively simulated with molecular dynamics simulations using the CHARMM36m force field. The results indicate that the alanine variant of the MTS preserves a uniform α-helical secondary structure favorable for the protein transport into mitochondria, whereas the valine variant quickly breaks down its α-helix. Thus, the alanine MTS represents the more active MnSOD variant, the benefits of which have yet to be determined experimentally. |
|---|
| Keywords: | manganese superoxide dismutase, polymorphism rs4880, mutation Ala16Val, molecular dynamics simulations, oxidative stress |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Submitted for review: | 20.10.2022 |
|---|
| Article acceptance date: | 25.11.2022 |
|---|
| Publication date: | 27.11.2022 |
|---|
| Publisher: | MDPI AG |
|---|
| Year of publishing: | 2022 |
|---|
| Number of pages: | 18 str. |
|---|
| Numbering: | Letn. 11, Št. 12 |
|---|
| PID: | 20.500.12556/DKUM-85181  |
|---|
| UDC: | 543 |
|---|
| ISSN on article: | 2076-3921 |
|---|
| COBISS.SI-ID: | 131249155 |
|---|
| DOI: | 10.3390/antiox11122348 |
|---|
| Copyright: | © 2022 by the authors |
|---|
| Publication date in DKUM: | 21.08.2023 |
|---|
| Views: | 568 |
|---|
| Downloads: | 25 |
|---|
| Metadata: |    |
|---|
| Categories: | Misc.
|
|---|
|
:
|
Copy citation |
|---|
| | | | Average score: | (0 votes) |
|---|
| Your score: | Voting is allowed only for logged in users. |
|---|
| Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
