| Naslov: | Optical detection of stress parameters : diploma thesis |
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| Avtorji: | ID Krstić, Marijana (Avtor) ID Bren, Urban (Mentor) Več o mentorju...  ID Lobnik, Aleksandra (Komentor) ID Sliesarenko, Valeriia V. (Komentor) |
| Datoteke: | UN_Krstic_Marijana_2023.pdf (2,07 MB) MD5: 1560087EF4D29BD493C2A7D92DA618ED
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Diplomsko delo/naloga |
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| Tipologija: | 2.11 - Diplomsko delo |
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| Organizacija: | FKKT - Fakulteta za kemijo in kemijsko tehnologijo
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| Opis: | In this thesis we developed the optical method of dopamine detection at pH 9, by creating DA-OPA complex. O–phthaldehyde was used as the indicator for fluorescent detection of dopamine with (3-Mercaptopropyl)-triethoxysilane as an additional reagent. The fluorescent product reached its maximum after 2 minutes, wavelengths of emission and excitation were λex/λem=340 nm/460 nm. The determination of dopamine was linear within a concentration range between 0.5∙10–6 and 3∙10–6 M. Limit of detection was 3.38∙10–6 M. Urea and norepinephrine can interfere dopamine detection, under examined parameters: ratio DA:OPA was 1:100, OPA:MPTES was 1:1, buffer was at pH 9. |
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| Ključne besede: | dopamine, fluorescence, o-phthaldehyde, optical method |
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| Kraj izida: | Maribor |
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| Kraj izvedbe: | Maribor |
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| Leto izida: | 2023 |
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| Št. strani: | 1 spletni vir (1 datoteka PDF (VIII, 36 f.)) |
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| PID: | 20.500.12556/DKUM-85461  |
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| UDK: | 543.426:577.17(043.2) |
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| COBISS.SI-ID: | 165411075  |
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| Datum objave v DKUM: | 13.09.2023 |
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| Število ogledov: | 381 |
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| Število prenosov: | 32 |
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| Metapodatki: |  |
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| Področja: | KTFMB - FKKT
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