| Title: | Optical detection of stress parameters : diploma thesis |
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| Authors: | ID Krstić, Marijana (Author)
ID Bren, Urban (Mentor) More about this mentor... 
ID Lobnik, Aleksandra (Comentor)
ID Sliesarenko, Valeriia V. (Comentor) |
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| Files: |  UN_Krstic_Marijana_2023.pdf (2,07 MB)
MD5: 1560087EF4D29BD493C2A7D92DA618ED
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| Language: | English |
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| Work type: | Bachelor thesis/paper |
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| Typology: | 2.11 - Undergraduate Thesis |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | In this thesis we developed the optical method of dopamine detection at pH 9, by creating DA-OPA complex. O–phthaldehyde was used as the indicator for fluorescent detection of dopamine with (3-Mercaptopropyl)-triethoxysilane as an additional reagent. The fluorescent product reached its maximum after 2 minutes, wavelengths of emission and excitation were λex/λem=340 nm/460 nm. The determination of dopamine was linear within a concentration range between 0.5∙10–6 and 3∙10–6 M. Limit of detection was 3.38∙10–6 M. Urea and norepinephrine can interfere dopamine detection, under examined parameters: ratio DA:OPA was 1:100, OPA:MPTES was 1:1, buffer was at pH 9. |
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| Keywords: | dopamine, fluorescence, o-phthaldehyde, optical method |
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| Place of publishing: | Maribor |
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| Place of performance: | Maribor |
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| Year of publishing: | 2023 |
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| Number of pages: | 1 spletni vir (1 datoteka PDF (VIII, 36 f.)) |
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| PID: | 20.500.12556/DKUM-85461 |
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| UDC: | 543.426:577.17(043.2) |
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| COBISS.SI-ID: | 165411075 |
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| Publication date in DKUM: | 13.09.2023 |
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| Views: | 383 |
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| Downloads: | 32 |
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| Metadata: |    |
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| Categories: | KTFMB - FKKT
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