| Title: | Study of gold nanoparticles conjugated with SARS-CoV-2 S1 spike protein fragments |
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| Authors: | ID Jelen, Žiga (Author)
ID Kovač, Janez (Author)
ID Rudolf, Rebeka (Author) |
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| Files: |  nanomaterials-13-02160-v2_(1).pdf (3,41 MB)
MD5: A441F9DB4632ECD180BFE7794B045175
 https://www.mdpi.com/2079-4991/13/15/2160
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FS - Faculty of Mechanical Engineering
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| Abstract: | This study reports on the successful conjugation of SARS-CoV-2 S1 spike protein fragments with gold nanoparticles (AuNPs) that were synthesised with Ultrasonic Spray Pyrolysis (USP). This method enables the continuous synthesis of AuNPs with a high degree of purity, round shapes, and the formation of a surface that allows various modifications. The conjugation mechanism of USP synthesized AuNPs with SARS-CoV-2 S1 spike protein fragments was investigated. A gel electrophoresis experiment confirmed the successful conjugation of AuNPs with SARS-CoV-2 S1 fragments indirectly. X-ray Photoelectron Spectroscopy (XPS) analysis confirmed the presence of characteristic O1s and N1s peaks, which indicated that specific binding between AuNPs and SARS-CoV-2 S1 spike protein fragments takes place via a peptide bond formed with the citrate stabiliser. This bond is coordinated to the AuNP’s surface and the N-terminals of the protein, with the conjugate displaying the expected response within a prototype LFIA test. This study will help in better understanding the behaviour of AuNPs synthesised with USP and their potential use as sensors in colorimetric or electrochemical sensors and LFIA tests. |
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| Keywords: | gold nanoparticles, ultrasonic spray pyrolysis, conjugation, SARS-CoV-2 S1, characterisation |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 29.06.2023 |
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| Article acceptance date: | 24.07.2023 |
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| Publication date: | 25.07.2023 |
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| Publisher: | MDPI |
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| Year of publishing: | 2023 |
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| Number of pages: | str. 1-14 |
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| Numbering: | Vol. 13, iss. 15, [article no.] 2160 |
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| Source: | https://www.mdpi.com/2079-4991/13/15/2160 |
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| PID: | 20.500.12556/DKUM-86524  |
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| UDC: | 669.2:620.3 |
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| ISSN on article: | 2079-4991 |
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| COBISS.SI-ID: | 159776515 |
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| DOI: | 10.3390/nano13152160 |
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| Publication date in DKUM: | 07.12.2023 |
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| Views: | 572 |
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| Downloads: | 40 |
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| Metadata: |    |
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| Categories: | Misc.
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