| Title: | The extraction process, separation, and identification of curcuminoids from turmeric curcuma longa |
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| Authors: | ID Slaček, Gal (Author)
ID Kotnik, Petra (Author)
ID Osmić, Azra (Author)
ID Postružnik, Vesna (Author)
ID Knez, Željko (Author)
ID Finšgar, Matjaž (Author)
ID Knez Marevci, Maša (Author) |
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| Files: |  Slacek-2023-The_Extraction_Process,_Separation.pdf (3,27 MB)
MD5: 57FCB00402AD2DF64DC5E36C79FFA48C
 https://www.mdpi.com/2304-8158/12/21/4000
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
MF - Faculty of Medicine
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| Abstract: | Turmeric Curcuma longa is a well-known spice with various health benefits, attributed primarily to curcumin. Soxhlet extraction, cold maceration, ultrasound-assisted extraction (UAE), and supercritical fluid extraction were performed, and the content of total phenols, proanthocyanidins, and antioxidants was analysed by UV/VIS spectrophotometry. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed to identify and quantify the curcumin content. Supercritical extracts had the highest total phenolic content (538.95 mg GA/100 g material), while the Soxhlet extracts had the highest content of proanthocyanidins (4.77 mg PAC/100 g of material). Extracts obtained by UAE and supercritical extraction have the highest antioxidant potential. Antioxidant activity measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) was 64.27% and 1750.32 mg Trolox per g dry weight by 2,2-azinobis(3-ethylbenzothiazoline 6 sulphonic acid) (ABTS(+center dot)) for the extract obtained by supercritical extraction. The UAE resulted in the highest amount of curcumin (1.91 mg curcumin/g material). A kinetic study showed that extraction yield in supercritical extracts decreased with increasing temperature and that the content of isolated curcumin was inversely proportional to solvent-to-feed ratio (S/F). The present study has confirmed that turmeric is an excellent source of antioxidants, such as curcumin, that play an important role in reducing cellular stress by neutralising free radicals. |
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| Keywords: | turmeric, curcumin, total phenols, proanthocyanidins, antioxidants |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 31.08.2023 |
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| Article acceptance date: | 30.10.2023 |
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| Publication date: | 01.11.2023 |
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| Publisher: | MDPI |
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| Year of publishing: | 2023 |
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| Number of pages: | Str. 14 |
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| Numbering: | Let. 12, Št. 21, št. članka 4000 |
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| PID: | 20.500.12556/DKUM-87025  |
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| UDC: | 577 |
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| ISSN on article: | 2304-8158 |
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| COBISS.SI-ID: | 171998211 |
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| DOI: | 10.3390/foods12214000 |
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| Publication date in DKUM: | 09.02.2024 |
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| Views: | 622 |
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| Downloads: | 111 |
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| Metadata: |    |
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| Categories: | Misc.
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