| Title: | Cationised fibre-based cellulose multi-layer membranes for sterile and high-flow bacteria retention and inactivation |
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| Authors: | ID Kokol, Vanja (Author)
ID Kos, Monika (Author)
ID Vivod, Vera (Author)
ID Gunde-Cimerman, Nina (Author) |
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| Files: |  Kokol-2023-Cationised_Fibre-Based_Cellulose_Mu.pdf (3,99 MB)
MD5: 38E86EF6D0E3901029C62ED56E811561
 https://doi.org/10.3390/membranes13030284
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FS - Faculty of Mechanical Engineering
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| Abstract: | Low-cost, readily available, or even disposable membranes in water purification or downstream biopharma processes are becoming attractive alternatives to expensive polymeric columns or filters. In this article, the potential of microfiltration membranes prepared from differently orientated viscose fibre slivers, infused with ultrafine quaternised (qCNF) and amino-hydrophobised (aCNF) cellulose nanofibrils, were investigated for capturing and deactivating the bacteria from water during vacuum filtration. The morphology and capturing mechanism of the single- and multi-layer structured membranes were evaluated using microscopic imaging and colloidal particles. They were assessed for antibacterial efficacy and the retention of selected bacterial species (Escherichia coli, Staphylococcus aureus, Micrococcus luteus), differing in the cell envelope structure, hydrodynamic biovolume (shape and size) and their clustering. The aCNF increased biocidal efficacy significantly when compared to qCNF-integrated membrane, although the latter retained bacteria equally effectively by a thicker multi-layer structured membrane. The retention of bacterial cells occurred through electrostatic and hydrophobic interactions, as well as via interfibrous pore diffusion, depending on their physicochemical properties. For all bacterial strains, the highest retention (up to 100% or log 6 reduction) at >50 L/h∗bar∗m2 flow rate was achieved with a 4-layer gradient-structured membrane containing different aCNF content, thereby matching the performance of industrial polymeric filters used for removing bacteria. |
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| Keywords: | fibrous membrane, cationised cellulose nanofibrils, amino-hydrophobised cellulose nanofibrils, antibacterial activity, multi-layer structure, flux, bacteria retention |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 30.01.2023 |
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| Article acceptance date: | 21.02.2023 |
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| Publication date: | 27.02.2023 |
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| Publisher: | MDPI |
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| Year of publishing: | 2023 |
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| Number of pages: | Str. 1-23 |
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| Numbering: | Letn. 13, Št. 3, št. članka 284 |
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| PID: | 20.500.12556/DKUM-87764  |
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| UDC: | 577:620.3 |
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| ISSN on article: | 2077-0375 |
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| COBISS.SI-ID: | 144515331 |
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| DOI: | 10.3390/membranes13030284 |
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| Publication date in DKUM: | 28.03.2024 |
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| Views: | 332 |
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| Downloads: | 18 |
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| Metadata: |    |
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| Categories: | Misc.
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