| Naslov: | The immobilization of β-galactosidase on glass fiber rolls |
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| Avtorji: | ID Pečar, Darja (Avtor)
ID Zečević, Katja (Avtor)
ID Goršek, Andreja (Avtor) |
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| Datoteke: |  catalysts-13-01503.pdf (2,84 MB)
MD5: DD0758120CB309B53831E83924CBB010
 https://www.mdpi.com/2073-4344/13/12/1503
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Članek v reviji |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FKKT - Fakulteta za kemijo in kemijsko tehnologijo
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| Opis: | The usability of glass fibers as immobilization support with a porous open structure was investigated. We developed a method to immobilize the enzyme β-galactosidase on special glass fiber rolls. The new method is simple, non-expensive and industrially applicable. Glutaraldehyde was used as a non-specific cross-linking agent for the covalent binding of β-galactosidase on modified glass fibers. The efficiency of immobilization was tested with the known hydrolysis of lactose. All experiments were performed in a continuous laboratory reactor. The influence of the reaction temperature (20, 25 and 30 °C), the substrate flow rate (1, 2 and 3 mL/min) and the pH of the reaction medium (6, 7 and 8) on the conversion was investigated. The reaction efficiency was monitored by measuring the glucose concentration with a spectrophotometer. High immobilization efficiency, enzyme activity and stability were obtained. The optimal reaction temperature, substrate flow rate and pH were found. The activity and stability of the enzyme entrapped on the glass fiber rolls remained almost unchanged during reuse, which is promising for potential industrial applications. |
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| Ključne besede: | beta-galaktosidase, immobilization, glass fiber, lactose |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 16.11.2023 |
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| Datum sprejetja članka: | 07.12.2023 |
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| Datum objave: | 09.12.2023 |
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| Založnik: | MDPI |
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| Leto izida: | 2023 |
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| Št. strani: | 11 str. |
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| Številčenje: | Vol. 13, no. 12, [article no.] 1503 |
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| PID: | 20.500.12556/DKUM-88340  |
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| UDK: | 66.02 |
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| COBISS.SI-ID: | 176801027 |
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| DOI: | 10.3390/catal13121503 |
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| ISSN pri članku: | 2073-4344 |
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| Datum objave v DKUM: | 17.04.2024 |
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| Število ogledov: | 363 |
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| Število prenosov: | 52 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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