| Title: | The immobilization of β-galactosidase on glass fiber rolls |
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| Authors: | ID Pečar, Darja (Author)
ID Zečević, Katja (Author)
ID Goršek, Andreja (Author) |
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| Files: |  catalysts-13-01503.pdf (2,84 MB)
MD5: DD0758120CB309B53831E83924CBB010
 https://www.mdpi.com/2073-4344/13/12/1503
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | The usability of glass fibers as immobilization support with a porous open structure was investigated. We developed a method to immobilize the enzyme β-galactosidase on special glass fiber rolls. The new method is simple, non-expensive and industrially applicable. Glutaraldehyde was used as a non-specific cross-linking agent for the covalent binding of β-galactosidase on modified glass fibers. The efficiency of immobilization was tested with the known hydrolysis of lactose. All experiments were performed in a continuous laboratory reactor. The influence of the reaction temperature (20, 25 and 30 °C), the substrate flow rate (1, 2 and 3 mL/min) and the pH of the reaction medium (6, 7 and 8) on the conversion was investigated. The reaction efficiency was monitored by measuring the glucose concentration with a spectrophotometer. High immobilization efficiency, enzyme activity and stability were obtained. The optimal reaction temperature, substrate flow rate and pH were found. The activity and stability of the enzyme entrapped on the glass fiber rolls remained almost unchanged during reuse, which is promising for potential industrial applications. |
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| Keywords: | beta-galaktosidase, immobilization, glass fiber, lactose |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 16.11.2023 |
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| Article acceptance date: | 07.12.2023 |
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| Publication date: | 09.12.2023 |
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| Publisher: | MDPI |
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| Year of publishing: | 2023 |
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| Number of pages: | 11 str. |
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| Numbering: | Vol. 13, no. 12, [article no.] 1503 |
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| PID: | 20.500.12556/DKUM-88340  |
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| UDC: | 66.02 |
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| ISSN on article: | 2073-4344 |
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| COBISS.SI-ID: | 176801027 |
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| DOI: | 10.3390/catal13121503 |
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| Publication date in DKUM: | 17.04.2024 |
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| Views: | 365 |
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| Downloads: | 52 |
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| Metadata: |    |
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| Categories: | Misc.
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