| Title: | Customized 16S-23S rDNA ITS amplicon metagenomics for acetic acid bacteria species identification in vinegarsand kombuchas |
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| Authors: | ID Ribič, Alja (Author)
ID Trček, Janja (Author) |
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| Files: |  RAZ_Ribic_Alja_2024.pdf (1,46 MB)
MD5: C4DEA2895B0CE96629B499E55CCA62E0
 https://www.mdpi.com/2076-2607/12/5/1023
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FNM - Faculty of Natural Sciences and Mathematics
FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | Acetic acid bacteria (AAB) are involved in food and beverage production bioprocesses, like
those in vinegar and kombucha. They oxidize sugars and alcohols into various metabolites, resulting
in the final products’ pleasant taste and aroma. The 16S rDNA amplicon metagenomics using
Illumina technology is usually used to follow the microbiological development of these processes.
However, the 16S rRNA gene sequences among different species of AAB are very similar, thus not
enabling a reliable identification down to the species level but only to the genus. In this study, we
have constructed primers for amplifying half of the 16S-23S rRNA gene internal transcribed spacer
(ITS) for library construction and further sequencing using Illumina technology. This approach was
successfully used to estimate the relative abundance of AAB species in defined consortia. Further
application of this method for the analysis of different vinegar and kombucha samples proves
it suitable for assessing the relative abundance of AAB species when these bacteria represent a
predominant part of a microbial community. |
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| Keywords: | ITS metagenomics, microbiota, consortia, Acetobacter, Komagataeibacter, Novacetimonas |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 04.05.2024 |
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| Article acceptance date: | 15.05.2024 |
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| Publication date: | 18.05.2024 |
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| Year of publishing: | 2024 |
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| Number of pages: | 8 str. |
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| Numbering: | Letn. 12, št. 5, št. članka 1023 |
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| PID: | 20.500.12556/DKUM-88782  |
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| UDC: | 579 |
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| ISSN on article: | 2076-2607 |
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| COBISS.SI-ID: | 196373763 |
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| DOI: | 10.3390/microorganisms12051023 |
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| Publication date in DKUM: | 04.02.2025 |
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| Views: | 172 |
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| Downloads: | 20 |
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| Metadata: |    |
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| Categories: | Misc.
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