Priprava organogelov z ekstrakti kurkume z optimalnim profilom sproščanja

Šiler, Nastja

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Title:	Priprava organogelov z ekstrakti kurkume z optimalnim profilom sproščanja
Authors:	ID Šiler, Nastja (Author) ID Knez Marevci, Maša (Mentor) More about this mentor... ID Žitek Makoter, Taja (Comentor)
Files:	MAG_Siler_Nastja_2024.pdf (3,29 MB) MD5: 70570394F09297EAEAF923C37235962B
Language:	Slovenian
Work type:	Master's thesis/paper
Typology:	2.09 - Master's Thesis
Organization:	FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:	Namen magistrskega dela je izolacija in določitev biološko aktivnih komponent (kurkuminoidov) v ekstraktih kurkume, formulacija ekstrahiranih učinkovin s ciljem čim večje vsebnosti kurkumina in preverjanje protimikrobnega potenciala dobljenih ekstraktov in formulacij. Izvedli smo dve različni ekstrakcijski metodi. Pri ultrazvočni ekstrakciji smo kot topilo uporabili etanol, medtem ko smo superkritično ekstrakcijo izvedli s superkritičnim ogljikovim dioksidom. Nastalim ekstraktom smo določili antioksidativno aktivnost z metodo DPPH· ter vsebnosti totalnih fenolov, proantocianidinov, karotenoidov in vitamina C. Ekstrakte smo nato formulirali v organogele in enkapsulate. Vsebnost kurkumina smo določili v ekstraktih in organogelih, njegovo topnost v superkritičnem ogljikovem dioksidu pa smo preverili z uporabo visokotlačne celice pri različnih tlakih. Na koncu je sledila še analiza protimikrobnega potenciala, s katero smo pripravljenim ekstraktom in formulacijam določili minimalno inhibitorno koncentracijo. Iz rezultatov je razvidno, da je najvišji ekstrakcijski izkoristek pri ultrazvočni ekstrakciji (5,77 %). Prav tako smo temu ekstraktu, v primerjavi s superkritičnim, spektrofotometrično določili višji % inhibicije (41,93 %) in višje vsebnosti totalnih fenolov (2,06 mg GA/g materiala), proantocianidinov (0,6 mg PAC/g materiala) ter vitamina C (0,20 mg vitamina C/g materiala). Tudi vsebnosti kurkumina so višje v ultrazvočnem ekstraktu. Ultrazvočni ekstrakt in njegove formulacije imajo nižjo minimalno inhibitorno koncentracijo kot superkritični ekstrakt in njegove formulacije. Najnižnjo minimalno inhibitorno koncentracijo (0,47 mg/mL) smo določili ultrazvočenmu ekstraktu pri uporabi bakterije Staphylococcus aureus in glive Candida albicans. S tem smo dodatno potrdili višje vsebnosti kurkumina v ultrazvočnem ekstraktu in tudi to, da kurkumin deluje protimikrobno.
Keywords:	kurkuma, kurkumin, ekstrakcija, organogeli, enkapsulacija, antimikrobni potencial
Place of publishing:	Maribor
Publisher:	[N. Šiler]
Year of publishing:	2024
PID:	20.500.12556/DKUM-89677
UDC:	66.061.3:633.826(043.2)
COBISS.SI-ID:	214505731
Publication date in DKUM:	10.09.2024
Views:	205
Downloads:	104
Metadata:
Categories:	KTFMB - FKKT
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Licences

License:	CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

Link:	http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:	The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:	26.08.2024

Secondary language

Language:	English
Title:	Formulation of organogels with turmeric extracts with optimal dissolution profile
Abstract:	The aim of this master thesis is the isolation and determination of biologically active components (curcruminoids) of turmeric extracts, the formulation of the extracted active compounds with the aim to maximise the curcumin content and to verify the antimicrobial potential of the produced extracts and formulations. Two different extraction methods were perfomed. In ultrasound extraction, ethanol was used as a solvent, while supercritical extraction was performed using supercritical carbon dioxide. The resulting extracts were analysed for their antioxidant activity using the DPPH method and determined for total phenols, proanthocyanidins, carotenoids and vitamin C. The extracts were then formulated into organogels and encapsulates. The curcumin content was determined in the extracts and organogels, and its solubility in supercritical carbon dioxide was tested using a high pressure cell at different pressures. Finally, an analysis of the antimicrobial potential of the prepared extracts and formulations was performed to determine the minimum inhibitory concentration. The results show that the maximum extraction yield is achieved with ultrasound extraction (5,77 %). We also spectrophotometrically determined that this extract, compared to the supercritical extract, has higher % inhibition (41,93 %) and higher content of total phenolics (2,06 mg GA/g materiala), proanthocyanidins (0,6 mg PAC/g materiala) and vitamin C (0,20 mg vitamina C/g materiala). The curcumin content is also higher in the ultrasound extracts. The ultrasound extract and its formulations have a lower minimum inhibitory concentration than the supercritical extract and its formulations. The lowest minimum inhibitory concentration (0,47 mg/mL) was determined for the ultrasound extract using Staphylococcus aureus and Candida albicans. Thus, we have further confirmed that the curcumin content is higher in the ultrasound extracts and that curcumin has antimicrobial activity.
Keywords:	turmeric, curcumin, extraction, organogels, encapsulation, antimicrobial potential

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