| Naslov: | Mesocellular silica foam as immobilization carrier for production of statin precursors |
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| Avtorji: | ID Skendrović, Dino (Avtor)
ID Primožič, Mateja (Avtor)
ID Rezić, Tonči (Avtor)
ID Vrsalović Presečki, Ana (Avtor) |
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| Datoteke: |  ijms-25-01971.pdf (6,28 MB)
MD5: DB64F0B3365DAA83B6EB1FA8C29A9A15
 https://www.mdpi.com/1422-0067/25/4/1971
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Članek v reviji |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FKKT - Fakulteta za kemijo in kemijsko tehnologijo
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| Opis: | The employment of 2-deoxyribose-5-phosphate aldolase (DERA) stands as a prevalent biocatalytic route for synthesizing statin side chains. The main problem with this pathway is the low stability of the enzyme. In this study, mesocellular silica foam (MCF) with different pore sizes was used as a carrier for the covalent immobilization of DERA. Different functionalizing and activating agents were tested and kinetic modeling was subsequently performed. The use of succinic anhydride as an activating agent resulted in an enzyme hyperactivation of approx. 140%, and the stability almost doubled compared to that of the free enzyme. It was also shown that the pore size of MCF has a decisive influence on the stability of the DERA enzyme. |
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| Ključne besede: | immobilization, aldolase, DERA, statins, mesoporous silica, pore size |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 16.01.0224 |
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| Datum sprejetja članka: | 03.02.2024 |
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| Datum objave: | 06.02.2024 |
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| Založnik: | MDPI |
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| Leto izida: | 2024 |
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| Št. strani: | 13 str. |
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| Številčenje: | Vol. 25, iss. 4, [article no.] 1971 |
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| PID: | 20.500.12556/DKUM-90460-db4af0c4-7829-0f52-d352-ed74908c17c2  |
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| UDK: | 620.3 |
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| COBISS.SI-ID: | 206319875 |
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| DOI: | 10.3390/ijms25041971 |
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| ISSN pri članku: | 1422-0067 |
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| Avtorske pravice: | © 2024 by the authors |
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| Datum objave v DKUM: | 04.09.2024 |
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| Število ogledov: | 223 |
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| Število prenosov: | 24 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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