| Naslov: | Dual mode of action of acetylcholine on cytosolic calcium oscillations in pancreatic beta and acinar cells in situ |
|---|
| Avtorji: | ID Sluga, Nastja (Avtor)
ID Postić, Sandra (Avtor)
ID Sarikas, Srdjan (Avtor)
ID Huang, Ya-Chi (Avtor)
ID Stožer, Andraž (Avtor)
ID Rupnik, Marjan (Avtor) |
|---|
| Datoteke: |  Sluga-2021-Dual_Mode_of_Action_of_Acetylcholin.pdf (2,41 MB)
MD5: F140052ACCE86C604686E7BCA11DAD5D
 https://doi.org/10.3390/cells10071580
|
|---|
| Jezik: | Angleški jezik |
|---|
| Vrsta gradiva: | Znanstveno delo |
|---|
| Tipologija: | 1.01 - Izvirni znanstveni članek |
|---|
| Organizacija: | MF - Medicinska fakulteta
|
|---|
| Opis: | Cholinergic innervation in the pancreas controls both the release of digestive enzymes to support the intestinal digestion and absorption, as well as insulin release to promote nutrient use in the cells of the body. The effects of muscarinic receptor stimulation are described in detail for endocrine beta cells and exocrine acinar cells separately. Here we describe morphological and functional criteria to separate these two cell types in situ in tissue slices and simultaneously measure their response to ACh stimulation on cytosolic Ca2+ oscillations [Ca2+]c in stimulatory glucose conditions. Our results show that both cell types respond to glucose directly in the concentration range compatible with the glucose transporters they express. The physiological ACh concentration increases the frequency of glucose stimulated [Ca2+]c oscillations in both cell types and synchronizes [Ca2+]c oscillations in acinar cells. The supraphysiological ACh concentration further increases the oscillation frequency on the level of individual beta cells, inhibits the synchronization between these cells, and abolishes oscillatory activity in acinar cells. We discuss possible mechanisms leading to the observed phenomena. |
|---|
| Ključne besede: | pancreas tissue slices, acetylcholine, beta cell, acinar cell, Ca2+ oscillations |
|---|
| Status publikacije: | Objavljeno |
|---|
| Verzija publikacije: | Objavljena publikacija |
|---|
| Poslano v recenzijo: | 14.05.2021 |
|---|
| Datum sprejetja članka: | 19.06.2021 |
|---|
| Datum objave: | 23.06.2021 |
|---|
| Založnik: | MDPI |
|---|
| Leto izida: | 2021 |
|---|
| Št. strani: | Str. 1-14 |
|---|
| Številčenje: | Letn. 10, št. 7, št. članka 1580 |
|---|
| PID: | 20.500.12556/DKUM-90985  |
|---|
| UDK: | 612.349.7 |
|---|
| COBISS.SI-ID: | 68553731 |
|---|
| DOI: | 10.3390/cells10071580 |
|---|
| ISSN pri članku: | 2073-4409 |
|---|
| Datum objave v DKUM: | 14.10.2024 |
|---|
| Število ogledov: | 187 |
|---|
| Število prenosov: | 25 |
|---|
| Metapodatki: |    |
|---|
| Področja: | Ostalo
|
|---|
|
:
|
Kopiraj citat |
|---|
| | | | Skupna ocena: | (0 glasov) |
|---|
| Vaša ocena: | Ocenjevanje je dovoljeno samo prijavljenim uporabnikom. |
|---|
| Objavi na: |  |
|---|
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše
podrobnosti ali sproži prenos. |
