| Title: | Isolation of live cells from different mice tissues up to nine days after death |
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| Authors: | ID Voga, Metka (Author)
ID Pleterski, Ana (Author)
ID Majdič, Gregor (Author) |
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| Files: |  Voga-2021-ISOLATION_OF_LIVE_CELLS_FROM_DIFFERE.pdf (1,48 MB)
MD5: 4267CF0D4B8B54B5143A3C84FBC47338
 https://doi.org/10.26873/SVR-1155-2021
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
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| Abstract: | Some limited reports suggest that cells can survive in the cadavers for much longer than it was previously thought. In our study we explored how time after death, tissue type (muscle, brain and adipose tissue), storage temperature of cadavers (4 °C or at room temperature) and form of tissue storage (stored as cadavers or tissue pieces in phosphate buffered saline) affect the success of harvesting live cells from mice after death. Cells were isolated from dead tissues and grown in standard conditions. Some cells were used for RNA extraction and RT² Profiler™ PCR Array for cell lineage identification was performed to establish which lineages the cells obtained from post mortem tissues belong to. Results of our study showed that viable cells can be regularly isolated from muscle and brain tissue 3 days post mortem and with difficulty up to 6 days post mortem. Viable cells from brain tissue can be isolated up to 9 days post mortem. No cells were isolated from adipose tissue except immediately after death. In all instances viable cells were isolated only when tissues were stored at 4 °C. Tissue storage did not affect cell isolation. Isolated cells were progenitors from different germ layers. Our results show that live cells could be obtained from mouse cadavers several days after death. |
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| Keywords: | mouse, cadaver, stem cells, brain, muscle, adipose tissue |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 19.09.2020 |
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| Article acceptance date: | 15.08.2021 |
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| Publication date: | 31.12.2021 |
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| Publisher: | Veterinarska fakulteta, Založba Univerze v Ljubljani |
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| Year of publishing: | 2021 |
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| Number of pages: | Str. 125-136 |
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| Numbering: | Letn. 58, št. 4 |
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| PID: | 20.500.12556/DKUM-91088  |
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| UDC: | 57.086:611.013.1:57.085.4:591.86+591.88:599.323.451 |
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| ISSN on article: | 1580-4003 |
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| COBISS.SI-ID: | 94280451 |
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| DOI: | 10.26873/SVR-1155-2021 |
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| Publication date in DKUM: | 21.10.2024 |
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| Views: | 116 |
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| Downloads: | 8 |
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| Metadata: |    |
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| Categories: | Misc.
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