| Title: | NMDA receptor inhibition increases, synchronizes, and stabilizes the collective pancreatic beta cell activity : insights through multilayer network analysis |
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| Authors: | ID Šterk, Marko (Author)
ID Križančić Bombek, Lidija (Author)
ID Skelin, Maša (Author)
ID Rupnik, Marjan (Author)
ID Marhl, Marko (Author)
ID Stožer, Andraž (Author)
ID Gosak, Marko (Author) |
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| Files: |  RAZ_Sterk_Marko_2021.pdf (4,64 MB)
MD5: 55F59448846EA2B0CCD868307024BAD1
 https://doi.org/10.1371/journal.pcbi.1009002
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
FNM - Faculty of Natural Sciences and Mathematics
PEF - Faculty of Education
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| Abstract: | NMDA receptors promote repolarization in pancreatic beta cells and thereby reduce glucose-stimulated insulin secretion. Therefore, NMDA receptors are a potential therapeutic target for diabetes. While the mechanism of NMDA receptor inhibition in beta cells is rather well understood at the molecular level, its possible effects on the collective cellular activity have not been addressed to date, even though proper insulin secretion patterns result from well-synchronized beta cell behavior. The latter is enabled by strong intercellular connectivity, which governs propagating calcium waves across the islets and makes the heterogeneous beta cell population work in synchrony. Since a disrupted collective activity is an important and possibly early contributor to impaired insulin secretion and glucose intolerance, it is of utmost importance to understand possible effects of NMDA receptor inhibition on beta cell functional connectivity. To address this issue, we combined confocal functional
multicellular calcium imaging in mouse tissue slices with network science approaches. Our results revealed that NMDA receptor inhibition increases, synchronizes, and stabilizes beta cell activity without affecting the velocity or size of calcium waves. To explore intercellular interactions more precisely, we made use of the multilayer network formalism by regarding each calcium wave as an individual network layer, with weighted directed connections portraying the intercellular propagation. NMDA receptor inhibition stabilized both the role of wave initiators and the course of waves. The findings obtained with the experimental antagonist of NMDA receptors, MK-801, were additionally validated with dextrorphan, the active metabolite of the approved drug dextromethorphan, as well as with experiments on NMDA receptor KO mice. In sum, our results provide additional and new evidence for a possible
role of NMDA receptor inhibition in treatment of type 2 diabetes and introduce the multilayer network paradigm as a general strategy to examine effects of drugs on connectivity in multicellular systems. |
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| Keywords: | pancreas, beta cells, insulin, Islets of Langerhans |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 06.11.2020 |
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| Article acceptance date: | 26.11.2021 |
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| Publication date: | 11.05.2021 |
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| Publisher: | Public Library of Science |
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| Year of publishing: | 2021 |
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| Number of pages: | str. 1-29 |
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| Numbering: | Letn. 17, št. 5 |
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| PID: | 20.500.12556/DKUM-91113  |
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| UDC: | 612.349.7 |
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| ISSN on article: | 1553-734X |
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| COBISS.SI-ID: | 63055107 |
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| DOI: | 10.1371/journal.pcbi.1009002 |
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| Publication date in DKUM: | 29.11.2024 |
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| Views: | 217 |
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| Downloads: | 10 |
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| Metadata: |    |
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| Categories: | Misc.
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