| Title: | Network representation of multicellular activity in pancreatic islets : Technical considerations for functional connectivity analysis |
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| Authors: | ID Šterk, Marko (Author)
ID Zhang, Yaowen (Author)
ID Pohorec, Viljem (Author)
ID Paradiž, Eva (Author)
ID Dolenšek, Jurij (Author)
ID Benninger, Richard K. P. (Author)
ID Stožer, Andraž (Author)
ID Kravets, Vira (Author)
ID Gosak, Marko (Author) |
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| Files: |  RAZ_Sterk_Marko_2024.pdf (4,48 MB)
MD5: 49939DCDA6303DCEDC499CC086910FEF
 http://dx.doi.org/10.1371/journal.pcbi.1012130
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
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| Abstract: | Within the islets of Langerhans, beta cells orchestrate synchronized insulin secretion, a pivotal aspect of metabolic homeostasis. Despite the inherent heterogeneity and multimodal activity of individual cells, intercellular coupling acts as a homogenizing force, enabling coordinated responses through the propagation of intercellular waves. Disruptions in this coordination are implicated in irregular insulin secretion, a hallmark of diabetes. Recently, innovative approaches, such as integrating multicellular calcium imaging with network analysis, have emerged for a quantitative assessment of the cellular activity in islets. However, different groups use distinct experimental preparations, microscopic techniques, apply different methods to process the measured signals and use various methods to derive functional connectivity patterns. This makes comparisons between findings and their integration into a bigger picture difficult and has led to disputes in functional connectivity interpretations. To address these issues, we present here a systematic analysis of how different approaches influence the network representation of islet activity. Our findings show that the choice of methods used to construct networks is not crucial, although care is needed when combining data from different islets. Conversely, the conclusions drawn from network analysis can be heavily affected by the pre-processing of the time series, the type of the oscillatory component in the signals, and by the experimental preparation. Our tutorial-like investigation aims to resolve interpretational issues, reconcile conflicting views, advance functional implications, and encourage researchers to adopt connectivity analysis. As we conclude, we outline challenges for future research, emphasizing the broader applicability of our conclusions to other tissues exhibiting complex multicellular dynamics. |
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| Keywords: | islets of Langerhans, beta cells, calcium signaling, intercellular communication, functional networks, myosin model |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 03.01.2024 |
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| Article acceptance date: | 02.05.2024 |
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| Publication date: | 13.05.2024 |
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| Publisher: | PLOS |
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| Year of publishing: | 2024 |
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| Number of pages: | Str. 1-33 |
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| Numbering: | Letn. 20, Št. 5 |
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| PID: | 20.500.12556/DKUM-91289  |
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| UDC: | 577 |
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| ISSN on article: | 1553-7358 |
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| COBISS.SI-ID: | 195539971 |
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| DOI: | 10.1371/journal.pcbi.1012130 |
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| Publication date in DKUM: | 09.12.2024 |
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| Views: | 151 |
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| Downloads: | 20 |
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| Metadata: |    |
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| Categories: | Misc.
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