| Title: | An overview of the current state of cell viability assessment methods using OECD classification |
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| Authors: | ID Madorran, Eneko (Author)
ID Ambrož, Miha (Author)
ID Knez, Jure (Author)
ID Sobočan, Monika (Author) |
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| Files: |  RAZ_Madorran_Eneko_2025.pdf (2,77 MB)
MD5: AF194C1F67EEFCB47BB756B395CC01E0
 https://doi.org/10.3390/ijms26010220
https://www.mdpi.com/1422-0067/26/1/220
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.02 - Review Article |
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| Organization: | MF - Faculty of Medicine
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| Abstract: | Over the past century, numerous methods for assessing cell viability have been
developed, and there are many different ways to categorize these methods accordingly. We
have chosen to use the Organisation for Economic Co-operation and Development (OECD)
classification due to its regulatory importance. The OECD categorizes these methods
into four groups: non-invasive cell structure damage, invasive cell structure damage, cell
growth, and cellular metabolism. Despite the variety of cell viability methods available,
they can all be categorized within these four groups, except for two novel methods based
on the cell membrane potential, which we added to the list. Each method operates on
different principles and has its own advantages and disadvantages, making it essential
for researchers to choose the method that best fits their experimental design. This review
aims to assist researchers in making this decision by describing these methods regarding
their potential use and providing direct references to the cell viability assessment methods.
Additionally, we use the OECD classification to facilitate potential regulatory use and to
highlight the need for adding a new category to their list. |
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| Keywords: | cell viability, cell-based methods, in vitro toxicology, OECD cell viability classification |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 27.12.2024 |
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| Article acceptance date: | 29.12.2024 |
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| Publication date: | 30.12.2024 |
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| Publisher: | MDPI |
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| Year of publishing: | 2025 |
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| Number of pages: | Str. 1-18 |
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| Numbering: | Letn. 26, Št. 1, št. članka 220 |
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| PID: | 20.500.12556/DKUM-91846  |
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| UDC: | 576 |
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| ISSN on article: | 1422-0067 |
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| COBISS.SI-ID: | 225295363 |
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| DOI: | 10.3390/ijms26010220 |
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| Publication date in DKUM: | 13.02.2025 |
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| Views: | 335 |
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| Downloads: | 17 |
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| Metadata: |    |
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| Categories: | Misc.
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