| Naslov: | Nisin-loaded gelatin microparticles for the enhanced bioactivity of bacterial nanocellulose |
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| Avtorji: | ID Hren, Maša (Avtor)
ID Trček, Janja (Avtor)
ID Šakanović, Aleksandra (Avtor)
ID Obradović, Hristina (Avtor)
ID Erdani-Kreft, Mateja (Avtor)
ID Hribernik, Silvo (Avtor)
ID Gorgieva, Selestina (Avtor) |
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| Datoteke: |  1-s2.0-S0141813025017520-main.pdf (7,52 MB)
MD5: 16291C1097AB41B57A218177D1A81688
 https://www.sciencedirect.com/science/article/pii/S0141813025017520?via%3Dihub
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Članek v reviji |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FS - Fakulteta za strojništvo
FERI - Fakulteta za elektrotehniko, računalništvo in informatiko
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| Opis: | Bacterial nanocellulose (BnC) is of immense importance in medicine, although its lack of bioactivity present intriguing issue. We propose a method to modify BnC with gelatin and nisin biomolecules, and explore their synergistic effect on the antimicrobial activity. Gelatin microparticles (without/with nisin loading) with a size ~0.5 μm and ~ 1.3 μm were prepared by spray drying and stabilised by dehydrothermal treatment. Modified BnC-based membranes supported the formation of biologically relevant minerals and were non-cytotoxic to human gingival fibroblast cells (HGF). The presence of gelatin microparticles improved the viability of HGF by approximately 20 %, due to the effect of gelatin alone, independent of the addition of nisin. BnC coated with a nisin/gelatin solution reduces the viability of HGF by about 20 %, but this negative effect is not observed by nisin coated gelatin microparticles. The cell viability of BnC membranes was above 90 % in both porcine and human urothelial cells. The antimicrobial activity study confirmed an inhibitory effect of membranes modified with nisin-coated microparticles or a gelatin/nisin solution against Staphylococcus aureus at a non-cytotoxic nisin dose (150 μg/mL). The study demonstrates the structural effects of gelatin and gelatin/nisin mixtures on the bioactivity of BnC and provides a rationale for the modification procedure. |
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| Ključne besede: | bacterial nanocellulose, gelatin, nisin, spray-drying, antimicrobial function, bioactivity |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 20.09.2024 |
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| Datum sprejetja članka: | 15.02.2025 |
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| Datum objave: | 17.02.2025 |
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| Založnik: | Elsevier |
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| Leto izida: | 2025 |
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| Št. strani: | 15 str. |
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| Številčenje: | Vol. 305, part 1, [article no.] 141203 |
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| PID: | 20.500.12556/DKUM-91992  |
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| UDK: | 577:543.2/.9 |
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| COBISS.SI-ID: | 228411907 |
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| DOI: | 10.1016/j.ijbiomac.2025.141203 |
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| ISSN pri članku: | 1879-0003 |
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| Avtorske pravice: | © 2025 The Authors |
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| Datum objave v DKUM: | 10.03.2025 |
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| Število ogledov: | 132 |
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| Število prenosov: | 18 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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