| Title: | Nisin-loaded gelatin microparticles for the enhanced bioactivity of bacterial nanocellulose |
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| Authors: | ID Hren, Maša (Author)
ID Trček, Janja (Author)
ID Šakanović, Aleksandra (Author)
ID Obradović, Hristina (Author)
ID Erdani-Kreft, Mateja (Author)
ID Hribernik, Silvo (Author)
ID Gorgieva, Selestina (Author) |
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| Files: |  1-s2.0-S0141813025017520-main.pdf (7,52 MB)
MD5: 16291C1097AB41B57A218177D1A81688
 https://www.sciencedirect.com/science/article/pii/S0141813025017520?via%3Dihub
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FS - Faculty of Mechanical Engineering
FERI - Faculty of Electrical Engineering and Computer Science
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| Abstract: | Bacterial nanocellulose (BnC) is of immense importance in medicine, although its lack of bioactivity present intriguing issue. We propose a method to modify BnC with gelatin and nisin biomolecules, and explore their synergistic effect on the antimicrobial activity. Gelatin microparticles (without/with nisin loading) with a size ~0.5 μm and ~ 1.3 μm were prepared by spray drying and stabilised by dehydrothermal treatment. Modified BnC-based membranes supported the formation of biologically relevant minerals and were non-cytotoxic to human gingival fibroblast cells (HGF). The presence of gelatin microparticles improved the viability of HGF by approximately 20 %, due to the effect of gelatin alone, independent of the addition of nisin. BnC coated with a nisin/gelatin solution reduces the viability of HGF by about 20 %, but this negative effect is not observed by nisin coated gelatin microparticles. The cell viability of BnC membranes was above 90 % in both porcine and human urothelial cells. The antimicrobial activity study confirmed an inhibitory effect of membranes modified with nisin-coated microparticles or a gelatin/nisin solution against Staphylococcus aureus at a non-cytotoxic nisin dose (150 μg/mL). The study demonstrates the structural effects of gelatin and gelatin/nisin mixtures on the bioactivity of BnC and provides a rationale for the modification procedure. |
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| Keywords: | bacterial nanocellulose, gelatin, nisin, spray-drying, antimicrobial function, bioactivity |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 20.09.2024 |
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| Article acceptance date: | 15.02.2025 |
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| Publication date: | 17.02.2025 |
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| Publisher: | Elsevier |
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| Year of publishing: | 2025 |
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| Number of pages: | 15 str. |
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| Numbering: | Vol. 305, part 1, [article no.] 141203 |
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| PID: | 20.500.12556/DKUM-91992  |
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| UDC: | 577:543.2/.9 |
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| ISSN on article: | 1879-0003 |
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| COBISS.SI-ID: | 228411907 |
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| DOI: | 10.1016/j.ijbiomac.2025.141203 |
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| Copyright: | © 2025 The Authors |
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| Publication date in DKUM: | 10.03.2025 |
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| Views: | 131 |
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| Downloads: | 18 |
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| Metadata: |    |
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| Categories: | Misc.
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