| Title: | Copper sulfate elicitation effect on biomass production, phenolic compounds accumulation, and antioxidant activity of Morus nigra L. stem node culture |
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| Authors: | ID Senekovič, Jan (Author)
ID Jelen, Špela (Author)
ID Urbanek Krajnc, Andreja (Author) |
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| Files: |  https://www.mdpi.com/2223-7747/14/5/766
 Senekovic-2025-Plants_Cooper.pdf (2,56 MB)
MD5: 4A433F723C382BC431749DBDD185FEE2
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKBV - Faculty of Agriculture and Life Sciences
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| Abstract: | Phenolic compounds are strong antioxidant and antibacterial agents with great pharmacological, medicinal, nutritional, and industrial value. The potential of Morus nigra in stem node culture was investigated for the production of phenolic compounds and their elicitation with CuSO4. Individual phenolic compounds in the samples were identified and quantified by using HPLC-PDA and HPLC-MS methods, while the content of total phenolic compounds, the content of total flavonoids, and the antioxidant activity of methanolic extracts were evaluated spectrophotometrically. The highest fresh and dry weights were obtained in plantlets treated with 0.5 mM CuSO4 for 42 days. The highest total phenolic content, total flavonoid content, and antioxidant activity of the extracts were determined in stem node cultures treated with 3 mM CuSO4 for 42 days. Under the latter conditions, the predominant representatives of the caffeoylquinic acids, p-coumaric acid derivatives, kaempferol derivatives, and quercetin derivatives also achieved the highest content. The most abundant phenolic compound in all samples was the chlorogenic acid. The nodal culture of M. nigra elicited with CuSO4 could potentially be used for the industrial production of phenolic compounds, especially caffeoylquinic acids. Moreover, considering the biochemical response to CuSO4 treatment and the ability to tolerate and accumulate copper, the potential application of M. nigra in phytoremediation is also highlighted. |
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| Keywords: | antioxidative potential, black mulberry, copper stress, elicitation of phenolics, flavonoids, in vitro culture, nodal culture, phenolic acids |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 22.02.2025 |
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| Article acceptance date: | 28.02.2025 |
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| Publication date: | 02.03.2025 |
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| Year of publishing: | 2025 |
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| Number of pages: | 38 str. |
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| Numbering: | Vol. 14, Iss. 5, [article no.] 766 |
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| PID: | 20.500.12556/DKUM-92101  |
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| UDC: | 581 |
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| ISSN on article: | 2223-7747 |
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| COBISS.SI-ID: | 228015363 |
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| DOI: | 10.3390/plants14050766 |
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| Publication date in DKUM: | 25.03.2025 |
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| Views: | 207 |
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| Downloads: | 16 |
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| Metadata: |    |
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| Categories: | Misc.
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