| Title: | Supercritical fluid and conventional extractions of high value-added compounds from pomegranate peels waste: production, quantification and antimicrobial activity of bioactive constituents |
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| Authors: | ID Kupnik, Kaja (Author)
ID Leitgeb, Maja (Author)
ID Primožič, Mateja (Author)
ID Postružnik, Vesna (Author)
ID Kotnik, Petra (Author)
ID Kučuk, Nika (Author)
ID Knez, Željko (Author)
ID Knez Marevci, Maša (Author) |
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| Files: |  plants-11-00928.pdf (2,02 MB)
MD5: 4862A0508402F429AEABBEDC37AF17E0
 https://www.mdpi.com/2223-7747/11/7/928
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
FS - Faculty of Mechanical Engineering
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| Abstract: | This study is focused on different extractions (Cold Maceration (CM), Ultrasonic Extraction
(UE), Soxhlet Extraction (SE) and Supercritical Fluid Extraction (SFE)) of bioactive compounds from
pomegranate (Punica Granatum L.) fruit peels using methanol, ethanol, and acetone as solvents in
conventional extractions and changing operating pressure (10, 15, 20, 25 MPa) in SFE, respectively.
The extraction yields, total phenols (TP) and proanthocyanidins (PAC) contents, and antioxidant
activity of different extracts are revealed. TP and PAC recovered by extracts ranged from 24.22 to
42.92 mg gallic acid equivalents (GAE)/g and 2.01 to 5.82 mg PAC/g, respectively. The antioxidant
activity of extracts ranged from 84.70% to 94.35%. The phenolic compound identification and
quantification in selective extracts was done using the LC-MS/MS method. The contents of different
flavonoids and phenolic acids have been determined. SFE extract, obtained at 20 MPa, contained
the highest content (11,561.84 µg/g) of analyzed total polyphenols, with predominant ellagic acid
(7492.53 µg/g). For the first time, Microbial Growth Inhibition Rates (MGIRs) were determined at
five different concentrations of pomegranate SFE extract against seven microorganisms. Minimal
Inhibitory Concentration (MIC90) was determined as 2.7 mg/mL of SFE pomegranate peel extract in
the case of five different Gram-negative and Gram-positive bacteria. |
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| Keywords: | antimicrobial activity, antioxidants, bioactive compounds, extraction, LC-MS/MS, phenolics, phytochemistry, pomegranate, Punica Granatum L., secondary metabolites |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 04.03.2022 |
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| Article acceptance date: | 28.03.2022 |
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| Publication date: | 30.03.2022 |
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| Publisher: | MDPI AG |
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| Year of publishing: | 2022 |
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| Number of pages: | 17 str. |
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| Numbering: | Vol. 11, iss. 7 |
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| PID: | 20.500.12556/DKUM-92246  |
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| UDC: | 66.02 |
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| ISSN on article: | 2223-7747 |
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| COBISS.SI-ID: | 102860803 |
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| DOI: | 10.3390/plants11070928 |
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| Copyright: | © 2022 by the authors |
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| Publication date in DKUM: | 24.03.2025 |
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| Views: | 167 |
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| Downloads: | 20 |
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| Metadata: |    |
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| Categories: | Misc.
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