| Title: | MFUM-BrTNBC-1, a newly established patient-derived triple-negative breast cancer cell line : molecular characterisation, genetic stability, and comprehensive comparison with commercial breast cancer cell lines |
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| Authors: | ID Skok, Kristijan (Author)
ID Gradišnik, Lidija (Author)
ID Čelešnik, Helena Sabina (Author)
ID Milojević, Marko (Author)
ID Potočnik, Uroš (Author)
ID Jezernik, Gregor (Author)
ID Gorenjak, Mario (Author)
ID Sobočan, Monika (Author)
ID Takač, Iztok (Author)
ID Kavalar, Rajko (Author)
ID Maver, Uroš (Author) |
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| Files: |  cells-11-00117-v2.pdf (5,33 MB)
MD5: D587CCC8173C9F1DB436AD6C1F60EF58
 https://www.mdpi.com/2073-4409/11/1/117
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
MF - Faculty of Medicine
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| Abstract: | Triple-negative breast cancer (TNBC) is a breast cancer (BC) subtype that accounts for
approximately 15–20% of all BC cases. Cancer cell lines (CLs) provide an efficient way to model the
disease. We have recently isolated a patient-derived triple-negative BC CL MFUM-BrTNBC-1 and
performed a detailed morphological and molecular characterisation and a comprehensive comparison
with three commercial BC CLs (MCF-7, MDA-MB-231, MDA-MB-453). Light and fluorescence
microscopy were used for morphological studies; immunocytochemical staining for hormone receptor,
p53 and Ki67 status; RNA sequencing, qRT-PCR and STR analysis for molecular characterisation; and
biomedical image analysis for comparative phenotypical analysis. The patient tissue-derived MFUMBrTNBC-1 maintained the primary triple-negative receptor status. STR analysis showed a stable and
unique STR profile up to the 6th passage. MFUM-BrTNBC-1 expressed EMT transition markers and
displayed changes in several cancer-related pathways (MAPK, Wnt and PI3K signalling; nucleotide
excision repair; and SWI/SNF chromatin remodelling). Morphologically, MFUM-BrTNBC-1 differed
from the commercial TNBC CL MDA-MB-231. The advantages of MFUM-BrTNBC-1 are its isolation
from a primary tumour, rather than a metastatic site; good growth characteristics; phenotype identical
to primary tissue; complete records of origin; a unique identifier; complete, unique STR profile;
quantifiable morphological properties; and genetic stability up to (at least) the 6th passage. |
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| Keywords: | hormonal receptors, MFUM-BrTNBC-1, MCF-7, MDA-MB-231, MDA-MB-453 |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 14.10.2021 |
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| Article acceptance date: | 27.12.2021 |
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| Publication date: | 30.12.2021 |
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| Publisher: | MDPI AG |
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| Year of publishing: | 2022 |
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| Number of pages: | str. [1]-23 |
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| Numbering: | Vol. 11, issue 1 |
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| PID: | 20.500.12556/DKUM-92461  |
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| UDC: | 618.19-006 |
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| ISSN on article: | 2073-4409 |
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| COBISS.SI-ID: | 91552259 |
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| DOI: | 10.3390/cells11010117 |
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| Copyright: | © 2021 by the authors |
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| Publication date in DKUM: | 10.04.2025 |
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| Views: | 209 |
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| Downloads: | 12 |
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| Metadata: |    |
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| Categories: | Misc.
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