| Title: | Natural Deep Eutectic Solvent-Based Matrix Solid Phase dispersion (MSPD) extraction for determination of bioactive compounds from sandy everlasting (Helichrysum arenarium L.) : a case of stability study |
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| Authors: | ID Ivanović, Milena (Author)
ID Krajnc, Peter (Author)
ID Mlinarič, Aleš (Author)
ID Islamčević Razboršek, Maša (Author) |
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| Files: |  plants-11-03468.pdf (2,47 MB)
MD5: 23B22B93EDAF0C7196E3419315B3DF37
 https://www.mdpi.com/2223-7747/11/24/3468
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | In the present study, vortex-assisted matrix solid-phase dispersion (VA-MSPD) extraction
was used to isolate the major bioactive compounds from H. arenarium. To reduce the negative environmental impact of the conventionally used organic solvents, four different choline chloride-based
natural deep eutectic solvents (NADES) were investigated as possible eluents. The most influential
VA-MSPD extraction parameters: stationary phase (adsorbent), adsorbent/sample ratio, vortex time,
and volume of extraction solvent were systematically optimized. Ultrasound-assisted extraction
with 80% MeOH was used as the standard method for the comparison of results. The stability of the
obtained extracts was studied over a period of 0 to 60 days at three different temperatures (−18 ◦C,
4
◦C, and 25 ◦C). All extracts were evaluated both spectrophotometrically (determination of total
phenolic content (TPC) and antioxidant activity by ABTS and FRAP assay) and chromatographically
(HPLC-UV). NADES based on choline chloride and lactic acid (ChCl-LA) was selected as the most
effective extractant, with a determined TPC value of its extract of 38.34 ± 0.09 mg GA/g DW (27%
higher than the methanolic VA-MSPD extract) and high antioxidant activity. The content of individual phenolic compounds (chlorogenic acid, dicaffeoylquinic acid isomers, naringenin isomers,
and chalcones) in the ChCl-LA extract, determined by HPLC-UV, was comparable to that of the
conventionally obtained one. Moreover, the stabilization effect of ChCl-LA was confirmed for the
studied compounds: chlorogenic acid, naringenin-40
-O-glucoside, tomoroside A, naringenin-5-Oglucoside, isosalipurposide, and naringenin. The optimum VA-MSPD conditions for the extraction of
H. arenarium polyphenols were: florisil/sample ratio of 0.5/1, a vortex time of 2 min, and an elution
volume of ChCl-LA of 10 mL. |
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| Keywords: | matrix solid phase dispersion extraction, MSPD, natural deep eutectic solvents, NADES, extraction optimization, sandy everlasting, phenolic compounds, stability studies, antioxidant activity |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 11.11.2022 |
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| Article acceptance date: | 06.12.2022 |
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| Publication date: | 11.12.2022 |
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| Publisher: | MDPI |
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| Year of publishing: | 2022 |
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| Number of pages: | 17 str. |
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| Numbering: | Vol. 11, iss. 24 |
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| PID: | 20.500.12556/DKUM-92750  |
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| UDC: | 66.02 |
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| ISSN on article: | 2223-7747 |
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| COBISS.SI-ID: | 134436867 |
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| DOI: | 10.3390/plants11243468 |
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| Copyright: | © 2022 by the authors |
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| Publication date in DKUM: | 09.05.2025 |
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| Views: | 264 |
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| Downloads: | 12 |
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| Metadata: |    |
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| Categories: | Misc.
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