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Title:Razvoj in vitro modela kože
Authors:ID Škrinjar, Dominik (Author)
ID Sever, Maja (Author)
ID Maver, Tina (Author)
ID Gradišnik, Lidija (Author)
ID Zidarič, Tanja (Author)
Files:.pdf Development_of_an_in_vitro_skin-Skrinjar-2024.pdf (5,78 MB)
MD5: 4E6017305BA5927FB0D4D54989C5B910
 
URL https://journals.um.si/index.php/anali-pazu/article/view/4436
 
URL https://www.dlib.si/details/URN:NBN:SI:DOC-XKLUQGFK
 
Language:Slovenian
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
UZUM - University of Maribor Press
Abstract:V raziskavi smo razvili biočrnilo za 3D tiskanje z vključitvijo človeških kožnih celic, sestavljeno iz alginata, metilceluloze in nanofibrilarne celuloze v celičnem mediju. 3D biotiskalnik omogoča ustvarjanje večslojnih ogrodij, ki simulirajo naravno celično mikrookolje kože. Zaradi potrebe po ponovljivosti smo biočrnilo hranili v zamrzovalniku in ga kasneje odtajali. Reološke lastnosti materiala po odtalitvi bistveno vplivajo na njegovo viskoznost, kar vpliva na možnost 3D tiskanja in stabilnost natisnjenih ogrodij. Ugotovili smo, da temperatura zamrzovanja pomembno vpliva na te lastnosti, kar je ključno za nadaljnji razvoj in vitro modela kože, ki je obetaven za predklinične in klinične raziskave.
Keywords:in vitro model kože, reologija, 3D tiskanje, predklinična medicina, kožne bolezni
Publication status:Published
Publication version:Version of Record
Submitted for review:07.03.2024
Article acceptance date:10.04.2024
Publication date:31.05.2024
Place of publishing:Maribor
Publisher:Univerza v Mariboru, Univerzitetna založba
Year of publishing:2024
Number of pages:19
Numbering:Letn.14, št.1
PID:20.500.12556/DKUM-93998 New window
UDC:616.5
ISSN on article:2820-364X
COBISS.SI-ID:197650947 New window
DOI:10.18690/analipazu.14.1.29-47.2024 New window
Publication date in DKUM:25.02.2026
Views:194
Downloads:11
Metadata:XML DC-XML DC-RDF
Categories:Misc.
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Record is a part of a journal

Title:Anali PAZU
Publisher:Združenje Pomurska akademsko znanstvena unija, Združenje Pomurska akademsko znanstvena unija, Univerzitetna založba Univerze v Mariboru
ISSN:2232-416X
COBISS.SI-ID:257553152 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:L7-4494-2022
Name:Kompleksen in vitro model kože z vključeno plastjo kosti za testiranje ne-invazivnega glukoznega senzorja

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P3-0036-2022
Name:Bio-psiho-socialni model kvalitete življenja

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

Language:English
Title:Development of an in Vitro Skin Model
Abstract:In this study, we developed bioink for 3D printing incorporating human skin cells, composed of alginate, methylcellulose, and nanofibrillar cellulose in a cell medium. The 3D bioprinter allows the creation of multilayer scaffolds that simulate the natural cellular microenvironment of the skin. Due to the need for reproducibility, we stored the bioink in a freezer and thawed it later. The rheological properties of the material after thawing significantly affect its viscosity, impacting the feasibility of 3D printing and the stability of the printed scaffolds. We found that the freezing temperature notably influences these properties, which is crucial for the further development of an in vitro skin model, promising for preclinical and clinical research.
Keywords:in vitro skin model, rheology, 3D printing, preclinical medicine, skin diseases


Collection

This document is a part of these collections:
  1. Anali PAZU

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