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Title:Vpliv ultrazvoka na izolacijo fenolnih spojin iz rožmarina (Rosmarinus Officinalis) : magistrsko delo
Authors:ID Caf, Nika (Author)
ID Škerget, Mojca (Mentor) More about this mentor... New window
ID Oman, Miha (Comentor)
Files:.pdf MAG_Caf_Nika_2025.pdf (3,14 MB)
MD5: 9CDF55F916BD328704C4CB880D521D26
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:V magistrskem delu smo preučevali vpliv ključnih procesnih parametrov na ultrazvočno ekstrakcijo bioaktivnih spojin iz rožmarina ter optimirali postopek za čim boljši izkoristek. Ultrazvočna ekstrakcija temelji na uporabi visokofrekvenčnih zvokov za ustvarjanje kavitacijskih mehurčkov v tekočini, kar vodi do mehanske desintegracije celičnih sten rastlinskih materialov in sproščanja bioaktivnih spojin. Pri ekstrakciji kavitacija, ki jo povzorči ultrazvočni val v topilu, časovno pospeši ekstrakcijo in poveča prenos snovi. Rožmarin (Rosmarinus officinalis) je aromatična rastlina, znana po svojem eteričnem olju, ki vsebuje številne bioaktivne spojine, kot so flavonoidi, triterpeni in karnozolna kislina, ki imajo antioksidativne, protivnetne in protimikrobne lastnosti. Naš cilj je bil ugotoviti, kako različni parametri, kot so razmerje med trdno in tekočo fazo, energija, amplituda, vrsta topila in število stopenj ekstrakcije, vplivajo na učinkovitost postopka in izkoristek pridobljenih spojin. Rezultati so pokazali, da povečanje energije sprva poveča izkoristek, vendar po določenem pragu dodatno povečevanje ni več učinkovito, saj se s povečanjem energije podaljša tudi čas ekstrakcije. Optimalen izkoristek smo dosegli z uporabo dveh stopenj ekstrakcije – začetno ultrazvočno obdelavo, ki ji sledi eno uro trajajoča ekstrakcija brez ultrazvoka. Opazili smo, da je tudi vrsta topila pomembno vplivala na izkoristek, pri čemer je bilo najuspešnejše topilo 1, medtem ko je bilo optimalno razmerje med trdnim materialom in topilom razmerje 4. S povečevanjem razmerja rožmarina in topila se je izkoristek povečeval. Pri preučevanju amplitude smo ugotovili, da je amplituda 2 najprimernejša, saj zagotavlja primerno ali željeno visoke izkoristke in hkrati omogoča direkten prenos postopka v industrijsko merilo. Pridobljeni razultati v sklopu magistrskega dela nakazujejo velik potencial uporabe, saj se lahko ekstrakcijski procesi z uporabo ultrazvoka do neke mere pospešijo. Čeprav se pričakuje, da se bomo pri prenosu tehnologije v industrijsko merilo srečali z manjšimi izzivi, pa prestavljajo rezultati te raziskave ključne smernice za nadaljni razvoj.
Keywords:rožmarin, ultrazvok, fenolne spojine, ultrazvočna ekstrakcija
Place of publishing:Maribor
Place of performance:Maribor
Publisher:[N. Caf]
Year of publishing:2025
Number of pages:1 spletni vir (1 datoteka PDF (X, 48 str.))
PID:20.500.12556/DKUM-95286 New window
UDC:66.061.3:582.943.16(043.2)
COBISS.SI-ID:255814659 New window
Publication date in DKUM:30.10.2025
Views:179
Downloads:31
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:11.09.2025

Secondary language

Language:English
Title:Effect of ultrasound on the isolation of phenolic compounds from rosemary (Rosmarinus Officinalis)
Abstract:In the master thesis, we studied the influence of the most important process parameters on the ultrasonic extraction of bioactive compounds from rosemary and how the process can be optimized for the best possible yield. Ultrasonic extraction is based on the use of high-frequency sounds to create cavitation bubbles in the liquid, leading to mechanical disintegration of the cell walls of the plant materials and the release of bioactive compounds. During extraction, the cavitation induced by the ultrasonic wave in the solvent accelerates the extraction over time and enhances the transfer of substances. Rosemary (Rosmarinus officinalis) is an aromatic plant known for its essential oil, which contains a number of bioactive compounds such as flavonoids, triterpenes and carnosic acid, that have antioxidant, anti-inflammatory and antimicrobial properties. The aim of this work was to determine how different parameters such as solid-liquid ratio, energy, amplitude, solvent type and extraction rates affect the efficiency of the process and the yield of the compounds obtained. The results showed that increasing the energy initially increases the yield, but after a certain threshold further increases are no longer effective, as increasing the energy also increases the extraction time. The optimum yield was achieved by two extraction steps - an initial ultrasonic treatment followed by an one-hour extraction without ultrasonic treatment. We observed that the type of solvent also had a significant effect on recovery, with solvent 1 performing the best, while the optimum solid to solvent ratio was 4. As the rosemary to solvent ratio increased, the recovery increased. When examining the amplitude, we found that an amplitude 2 was the most preferred amplitude, as it provides high efficiencies while allowing direct transfer to industrial scale. The results obtained as part of the MSc thesis indicate great potential for application, as the extraction processes with ultrasound can be accelerated to a certain extent. Although it is to be expected that we will encounter minor challenges when transferring the technology to an industrial scale, the results of this research provide important guidelines for further development.
Keywords:rosemary, ultrasound, phenolic compounds, ultrasound asissted extraction


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