| Title: | Chemo-enzymatic synthesis of viscoelastic pseudopeptidoglycan networks |
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| Authors: | ID Loibner, Philipp (Author)
ID Bučak-Gasser, David (Author)
ID Schober, Katharina (Author)
ID Steindorfer, Tobias Alexander (Author)
ID Brandtner, Monika (Author)
ID Dorn, Tobias (Author)
ID Wrodnigg, Tanja (Author)
ID Neshchadin, Dmytro (Author)
ID Gescheidt-Demner, Georg (Author)
ID Bračič, Matej (Author)
ID Lackner, Florian (Author)
ID Mohan, Tamilselvan (Author)
ID Stana-Kleinschek, Karin (Author)
ID Kargl, Rupert (Author) |
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| Files: |  chemo-enzymatic-synthesis-of-viscoelastic-pseudopeptidoglycan-networks.pdf (4,32 MB)
MD5: 439AEC2216CF31FD3F1B8F94B1394C94
 https://pubs.acs.org/doi/10.1021/acs.bioconjchem.5c00014
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| Language: | English |
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| Work type: | Article |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FS - Faculty of Mechanical Engineering
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| Abstract: | Bacterial peptidoglycans (PGs) are essential targets for antibiotics and immune cells. Chemical methods to reproduce PGs semisynthetically are tedious and wasteful. In this work, we describe a new approach to form pseudo-PGs (PPGs) using the protease papain and custom-made peptides conjugated to a glycan. The kinetics of formation is monitored by rheology and 1H NMR. Viscoelastic gels of controlled strength are formed, depending on the temperature and the number of peptide bridges between the glycan chains. We propose that the new method has a high impact on biomaterials research, since it could be used to deliver peptides, test antibiotic efficacy, or investigate human immune cell response. |
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| Keywords: | biomaterials, bacterial peptidoglycans (PGs), chemo-enzymatic synthesis |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 24.06.2025 |
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| Publisher: | ACS Publications |
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| Year of publishing: | 2025 |
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| Number of pages: | str. 1933-1942 |
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| Numbering: | Vol. 36, no. 9 |
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| PID: | 20.500.12556/DKUM-95867  |
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| UDC: | 576:60 |
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| ISSN on article: | 1520-4812 |
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| COBISS.SI-ID: | 254908163 |
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| DOI: | 10.1021/acs.bioconjchem.5c00014 |
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| Publication date in DKUM: | 03.11.2025 |
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| Views: | 130 |
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| Downloads: | 12 |
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| Metadata: |    |
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| Categories: | Misc.
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