Testiranje različnih pristopov zasnove elektrokemijskih MIP-senzorjev za določanje N-nitrozodimetilamina : magistrsko delo

Kolšek, Gaja

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Title:	Testiranje različnih pristopov zasnove elektrokemijskih MIP-senzorjev za določanje N-nitrozodimetilamina : magistrsko delo
Authors:	ID Kolšek, Gaja (Author) ID Finšgar, Matjaž (Mentor) More about this mentor... ID Rajh, Barbara (Comentor)
Files:	MAG_Kolsek_Gaja_2026.pdf (4,85 MB) MD5: 6D34494C8E7F599A4028160BAEC1E847
Language:	Slovenian
Work type:	Master's thesis/paper
Typology:	2.09 - Master's Thesis
Organization:	FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:	V tem delu smo preučevali uporabo elektrokemijskih senzorjev na osnovi molekularno vtisnjenih polimerov (MIP) za določanje N nitrozodimetilamina (NDMA). Pripravili smo MIP senzorje z različnimi pristopi zasnove z uporabo elektrode iz steklastega ogljika in zlate elektrode ter določili njihov elektrokemijski signal. MIP senzorje smo pripravili z dvostopenjskim postopkom, z in situ elektropolimerizacijo in in situ elektropolimerizacijo ob predhodnem nanosu nanodelcev pruskega modrila (PBNP). Kot analizni tehniki smo uporabili diferenčno pulzno voltametrijo in angl. square wave voltametrijo v prisotnosti redoks mediatorja [Fe(CN)6]3–/4–. Preverjali smo spreminjanje elektrokemijskega signala MIP senzorja z naraščajočo koncentracijo NDMA. Možnost večkratne uporabe MIP senzorja smo testirali z zaporednimi meritvami po inkubaciji in regeneraciji senzorja. Za primerjavo smo testirali tudi večkratno zaporedno uporabo elektrode brez MIP plasti. MIP temeljijo na polimerizaciji v prisotnosti matrične molekule, pri čemer po njeni odstranitvi nastanejo selektivna vezavna mesta, komplementarna ciljnemu analitu. Elektrokemijski MIP senzorji predstavljajo obetaven pristop za selektivno določanje, vendar kot je prikazano v tem delu elektrokemijski signal ni bil odvisen izključno od vezave analita. Tudi angl. non imprinted polymer senzor, pripravljen z in situ elektropolimerizacijo brez prisotnosti matrične molekule in nanosom PBNP ter elektrode brez MIP plasti ne kažejo analitske uporabnosti za analizo NDMA. Višina vrha se je pri zaporednih meritvah pri enaki koncentraciji postopno zmanjševala, regeneracija pa ni povrnila začetnega elektrokemijskega signala. Rezultati kažejo, da na elektrokemijski signal poleg vezave analita pomembno vplivajo tudi procesi na površini elektrode in v polimerni plasti, kar je treba upoštevati pri razvoju MIP senzorjev za določanje NDMA.
Keywords:	NDMA, molekularno vtisnjeni polimeri, elektrokemijski senzorji, voltametrija, analiza sledov
Place of publishing:	Maribor
Place of performance:	Maribor
Publisher:	[G. Kolšek]
Year of publishing:	2026
Number of pages:	1 spletni vir (1 datoteka PDF (XIII, 47 str.))
PID:	20.500.12556/DKUM-97704
UDC:	543.552:547.331(043.2)
COBISS.SI-ID:	279038467
Publication date in DKUM:	06.05.2026
Views:	204
Downloads:	52
Metadata:
Categories:	KTFMB - FKKT
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Licences

License:	CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

Link:	http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:	The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:	03.04.2026

Secondary language

Language:	English
Title:	Testing different approaches to design an electrochemical MIP sensor for the determination of N-nitrosodimethylamine
Abstract:	In this work, the application of electrochemical sensors based on molecularly imprinted polymers (MIPs) for the determination of N nitrosodimethylamine (NDMA) was investigated. MIP sensors were prepared using different approaches to sensor design on glassy carbon electrode and gold electrode, and their electrochemical signals were evaluated. MIP sensors were prepared using a two step procedure, in situ electropolymerization and in situ electropolymerization following the prior deposition of Prussian blue nanoparticles (PBNP). Differential pulse voltammetry and square wave voltammetry were employed as analytical techniques in the presence of the redox mediator [Fe(CN)6]3–/4–. The change in the electrochemical signal of the MIP sensor was followed as the NDMA concentration was increased. The reusability of the MIP sensor was tested by performing a series of measurements after incubation and regeneration. For comparison, electrodes without MIP films were also tested. MIPs are based on polymerization in the presence of a template molecule, in which, after its removal, selective binding sites complementary to the target analyte are formed. Electrochemical MIP sensors represent a promising approach for the selective determination of analytes. However, in this work, the electrochemical signal was not solely governed by the selective binding of the analyte. Likewise, the non-imprinted polymer sensor prepared by in situ electropolymerization in the absence of template and PBNP deposition, as well as electrodes without MIP films, do not exhibit analytical applicability for NDMA determination. The peak current gradually decreased during successive measurements at constant analyte concentration, and regeneration did not restore the initial electrochemical signal. These findings indicate that, in addition to analyte binding, processes at the electrode surface and within the MIP film significantly affect the electrochemical signal. This should be carefully considered in the development of MIP based sensors for NDMA determination.
Keywords:	NDMA, molecularly imprinted polymers, electrochemical sensors, voltammetry, trace analysis

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