| Title: | Disodium guanosine 5'-monophosphate self-associates into nanoscale cylinders at pH 8: a combined diffusion NMR spectroscopy and dynamic light scattering study |
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| Authors: | ID Wong, Alan (Author)
ID Ramsey, Ida (Author)
ID Spindler, Lea (Author)
ID Wu, Gang (Author) |
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| Files: |  http://dx.doi.org/10.1021/ja042794d
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| Language: | English |
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| Work type: | Unknown |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FS - Faculty of Mechanical Engineering
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| Abstract: | We report a combined NMR and dynamic light scattering (DLS) study on the size of supramolecular structures formed by disodium guanosine 5'-monophosphate, Na2(5'-GMP), at pH 8. In general, two distinct types of aggregate species are present in an aqueous solution of Na2(5'-GMP). One type consists of stacking 5'-GMP monomers, and the other contains stacking G-quartets. Both types of aggregates can be modeled as rodlike cylinders. The cylinder diameter is 10 and 26 A for monomer aggregates and quartet aggregates, respectively. For Na2(5'-GMP) concentrations between 18 and 34 wt %, the cylinders formed by stacking G-quartets have an average length between 8 and 30 nm, corresponding to a stack of ~24-87 G-quartets. These nanoscale aggregates are significantly larger than what had previously been believed for Na2(5'-GMP) self-associationat pH 8. The length of both types of 5'-GMP aggregates was found to increase with Na2(5ć-GMP) concentration but was insensitive to the added NaCl in solution. While the aggregate size for monomer aggregates increases with a decrease in temperature, the size of G-quartet aggregates is essentially independent of temperature. We found that the size of G-quartet aggregates is slightly larger in D2O than in H2O, whereas the size of monomer aggregates remains the same in D2O and in H2O. We observed a linear relationship between the axial ratio of the 5ć-GMP cylinders and the Na2(5'-GMP) concentration for both types of 5ć-GMP aggregates, which suggests a common stacking mechanism for monomers and G-quartets. |
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| Year of publishing: | 2005 |
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| PID: | 20.500.12556/DKUM-27047  |
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| UDC: | 539:544 |
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| ISSN on article: | 0002-7863 |
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| COBISS.SI-ID: | 9783574 |
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| NUK URN: | URN:SI:UM:DK:QRDD4OAF |
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| Publication date in DKUM: | 01.06.2012 |
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| Views: | 1838 |
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| Downloads: | 130 |
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| Metadata: |    |
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| Categories: | Misc.
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