| Naslov: | Disodium guanosine 5'-monophosphate self-associates into nanoscale cylinders at pH 8: a combined diffusion NMR spectroscopy and dynamic light scattering study |
|---|
| Avtorji: | ID Wong, Alan (Avtor)
ID Ramsey, Ida (Avtor)
ID Spindler, Lea (Avtor)
ID Wu, Gang (Avtor) |
|---|
| Datoteke: |  http://dx.doi.org/10.1021/ja042794d
|
|---|
| Jezik: | Angleški jezik |
|---|
| Vrsta gradiva: | Neznano |
|---|
| Tipologija: | 1.01 - Izvirni znanstveni članek |
|---|
| Organizacija: | FS - Fakulteta za strojništvo
|
|---|
| Opis: | We report a combined NMR and dynamic light scattering (DLS) study on the size of supramolecular structures formed by disodium guanosine 5'-monophosphate, Na2(5'-GMP), at pH 8. In general, two distinct types of aggregate species are present in an aqueous solution of Na2(5'-GMP). One type consists of stacking 5'-GMP monomers, and the other contains stacking G-quartets. Both types of aggregates can be modeled as rodlike cylinders. The cylinder diameter is 10 and 26 A for monomer aggregates and quartet aggregates, respectively. For Na2(5'-GMP) concentrations between 18 and 34 wt %, the cylinders formed by stacking G-quartets have an average length between 8 and 30 nm, corresponding to a stack of ~24-87 G-quartets. These nanoscale aggregates are significantly larger than what had previously been believed for Na2(5'-GMP) self-associationat pH 8. The length of both types of 5'-GMP aggregates was found to increase with Na2(5ć-GMP) concentration but was insensitive to the added NaCl in solution. While the aggregate size for monomer aggregates increases with a decrease in temperature, the size of G-quartet aggregates is essentially independent of temperature. We found that the size of G-quartet aggregates is slightly larger in D2O than in H2O, whereas the size of monomer aggregates remains the same in D2O and in H2O. We observed a linear relationship between the axial ratio of the 5ć-GMP cylinders and the Na2(5'-GMP) concentration for both types of 5ć-GMP aggregates, which suggests a common stacking mechanism for monomers and G-quartets. |
|---|
| Leto izida: | 2005 |
|---|
| PID: | 20.500.12556/DKUM-27047  |
|---|
| UDK: | 539:544 |
|---|
| COBISS.SI-ID: | 9783574 |
|---|
| ISSN pri članku: | 0002-7863 |
|---|
| NUK URN: | URN:SI:UM:DK:QRDD4OAF |
|---|
| Datum objave v DKUM: | 01.06.2012 |
|---|
| Število ogledov: | 1839 |
|---|
| Število prenosov: | 130 |
|---|
| Metapodatki: |    |
|---|
| Področja: | Ostalo
|
|---|
|
:
|
Kopiraj citat |
|---|
| | | | Skupna ocena: | (0 glasov) |
|---|
| Vaša ocena: | Ocenjevanje je dovoljeno samo prijavljenim uporabnikom. |
|---|
| Objavi na: |  |
|---|
Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše
podrobnosti ali sproži prenos. |
