| Title: | Response of monocyte-derived dendritic cells to rapidly solidified nickel-titanium ribbons with shape memory properties |
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| Authors: | ID Tomić, Sergej (Author)
ID Rudolf, Rebeka (Author)
ID Brunčko, Mihael (Author)
ID Anžel, Ivan (Author)
ID Savić, V. (Author)
ID Čolić, Miodrag (Author) |
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| Files: |  European_Cells_and_Materials_2012_Tomic_et_al._Response_of_monocyte-derived_dendritic_cells_to_rapidly_solidified_nickel-titanium_ribbon.pdf (1,35 MB)
MD5: 46D6B99513E650ADE5A0C66E33163A24
PID: 20.500.12556/dkum/8794315e-04c0-4719-a625-4e4aab07621e
 http://www.ecmjournal.org/journal/papers/vol023/vol023a05.php
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FS - Faculty of Mechanical Engineering
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| Abstract: | Ni-Ti Shape Memory Alloys (SMAs) have attracted considerable attention as biomaterials for medical devices. However, the biocompatibility of Ni-Ti SMAs is often unsatisfactory due to their poor surface structure. Here we prepared Rapidly Solidified (RS) Ni-Ti SMA ribbons by melt-spinning and their surface was characterised by Augerelectron spectroscopy, X-ray photoelectron spectrometry and scanning electron microscopy. The biocompatibility of the produced ribbons and their immunomodulatory properties were studied on human monocyte-derived dendritic cells (MoDCs). We showed that melt-spinning of Ni-Ti SMAs can form a thin homogenous oxide layer, which improves their corrosion resistance and subsequent toxicity to MoDCs. Ni-Ti RS ribbons stimulated the maturation of MoDCs, as detected by changes in the cells' morphology and increased expression of HLA-DR, CD86, CD40 and CD83 molecules. However, Ni-Ti RS ribbons enhanced the tolerogenic properties of immature MoDCs, which produced higher levels of IL-10 and IL-27, driving the differentiation of IL-10- and TGF-β-producing CD4+T cells. On the other hand, in the presence of lipopolysaccharide, an important pro-inflammatory biomolecule, Ni-Ti RS ribbons enhanced the allostimulatory and Th1 polarising capacity of MoDCs, whereas the production of Th2 and Th17 cytokines was down-regulated. In conclusion, Ni-Ti RS ribbons possess substantial immunomodulatory properties on MoDCs. These findings might be clinically relevant, because implanted Ni-Ti SMA devices can induce both desired and adverse effects on the immune system, depending on the microenvironmental stimuli. |
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| Keywords: | nickel-titanium alloy, biocompatibility, cytokines, immunomodulation, monocyte-derived dendritic cells |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2012 |
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| Number of pages: | str. 58-80 |
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| Numbering: | Letn. 23 |
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| PID: | 20.500.12556/DKUM-27240-d1276669-88df-d96d-7735-8604d2a7f30a  |
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| ISSN: | 1473-2262 |
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| UDC: | 669:61 |
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| ISSN on article: | 1473-2262 |
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| COBISS.SI-ID: | 15705366 |
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| DOI: | 10.22203/eCM.v023a05 |
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| NUK URN: | URN:SI:UM:DK:PQFJBRAW |
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| Publication date in DKUM: | 01.06.2012 |
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| Views: | 2292 |
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| Downloads: | 495 |
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| Metadata: |    |
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| Categories: | Misc.
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