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Title:UPORABA MEHANSKIH SIL ZA IZOLACIJO ENCIMOV IN PROTEINOV IZ CELIC ČRNE KVASOVKE TRIMMATOSTROMA SALINUM
Authors:ID Grobelnik, Kaja (Author)
ID Primožič, Mateja (Mentor) More about this mentor... New window
ID Leitgeb, Maja (Comentor)
Files:.pdf UNI_Grobelnik_Kaja_2012.pdf (2,39 MB)
MD5: 82E7371E8C88A54FB69CB845F090DB44
PID: 20.500.12556/dkum/a0f6351a-38ef-4c84-a76b-990ed9f40cac
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Namen diplomske naloge je bil, preučiti vpliv mehanskih sil na izločanje encimov in proteinov iz celic Trimmatostroma salinum pri atmosfereskem tlaku, 35 °C in različnih časih izpostavitve. Rezultati so pokazali, da se je s podaljševanjem časa homogenizacije preživelost celic Trimmatostroma salinum zmanjševala. Določevali smo tudi aktivnost β-glukozidaze, α-amilaze, proteaze, celulaze, ter koncentracijo proteinov pri 595 nm in 280 nm ter merili absorbanco pri 260 nm in 280 nm za določevanje prisotnosti nukleinskih kislin z UV-Vis spektrofotometru. S podaljševanjem časa homogenizacije smo dosegli, da so se iz celic T. salinum izločili intracelularni encimi.
Keywords:T. salinum, homogeniziranje, celulaza, proteza, β-glukozidaza, α-amilaza
Place of publishing:Maribor
Publisher:[K. Grobelnik]
Year of publishing:2012
PID:20.500.12556/DKUM-38589 New window
UDC:579.234:579.26(043.2)
COBISS.SI-ID:16682518 New window
NUK URN:URN:SI:UM:DK:9D4DWH8S
Publication date in DKUM:16.10.2012
Views:2269
Downloads:258
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Secondary language

Language:English
Title:USE OF MECHANICAL FORCES FOR ISOLATION OF ENZYMES AND PROTEINS FROM CELLS OF BLACK YEAST TRIMMATOSTROMA SALINUM
Abstract:The purpose of the diploma thesis was to examine how the secretion of enzymes and proteins are affected by mechanical forces with the extension of homogenization time at 35 °C and atmospheric pressure. The results showed that the survival of Trimmatostroma salinum cells decreased with prolongation of homogenization time at 35 °C. The activity of β-glucosidase, α-amylase, protease, cellulase, concentration of protein at 595 nm and 280 nm, and nucleic acids at 260 nm on UV-Vis spectrophotometer were also measured. With the increase of homogenization time more intracellular enzymes from suspension T. salinum cells were extracted.
Keywords:T. salinum, homogenization, cellulase, protease, β-glucosidase, α-amylase


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