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Title:VALIDACIJA METODE ZA DOLOČANJE AFLATOKSINOV V OREŠČKIH S TEKOČINSKO KROMATOGRAFIJO Z UPORABO IMUNOAFINITETNIH KOLON
Authors:ID Bojanc, Branka (Author)
ID Brodnjak-Vončina, Darinka (Mentor) More about this mentor... New window
ID Kolar, Mitja (Comentor)
ID Jeretin, Bogdana (Comentor)
Files:.pdf UNI_Bojanc_Branka_2013.pdf (20,50 MB)
MD5: BA980E8335E5F81561B50EE2B9BB8D28
PID: 20.500.12556/dkum/be8f4eff-d76f-4a06-9a2f-7cd51f020168
 
Language:Slovenian
Work type:Undergraduate thesis
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Za določanje aflatoksinov G1, G2, B1, B2 v oreščkih smo validirali metodo določanja s tekočinsko kromatografijo z uporabo imunoafinitetnih kolon. Rezultate analiziranih vzorcev smo opredelili s pomočjo umeritvene krivulje v koncentracijah od 1 μg/kg do 10 μg/kg. Dovoljena vsebnost vsote aflatoksinov G1, G2, B1, B2 v oreščkih je 4,0 μg/kg. Rezultate smo ovrednotili glede na predpisane zahteve analizne metode (koncentracijsko območje, meja določanja, meja zaznavnosti, ponovljivost, obnovljivost in pravilnost). Validirana metoda za določanje aflatoksinov G1, G2, B1, B2 v oreščkih s tekočinsko kromatografijo z uporabo imunoafinitetnih kolon je primerna in uporabna, kar smo potrdili z uspešnim sodelovanjem v medlaboratorijskim testom.
Keywords:aflatoksini, oreščki, validacija, tekočinska kromatografija
Place of publishing:Maribor
Publisher:[B. Bojanc]
Year of publishing:2013
PID:20.500.12556/DKUM-40472 New window
UDC:543.544:631.576.2(043.2)
COBISS.SI-ID:17473814 New window
NUK URN:URN:SI:UM:DK:CRSGPMRF
Publication date in DKUM:29.05.2013
Views:2675
Downloads:321
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Secondary language

Language:English
Title:METHOD VALIDATION FOR AFLATOXINS DETERMINATION IN NUTS WITH LIQUID CHROMATOGRAPHY USING IMMUNOAFFINITY COLUMNS
Abstract:For the determination of aflatoxin G1, G2, B1, B2 in the nuts using liquid chromatography with immunoaffinity columns method validation was performed. The results of the samples were determined using the calibration curve at concentration range from 1 μg/kg to 10 μg/kg. Permissible content of the sum of aflatoxins G1, G2, B1, B2 in nuts is 4.0 μg/kg. The results were evaluated according to the requirements of the analytical method (concentration range, limit of quantification, limit of detection, repeatability, reproducibility and accuracy). With the results of interlaboratory comparison it is confirmed that the proposed method is appropriate for the determination of aflatoxins G1, G2, B1, B2 in the nuts.
Keywords:aflatoxins, nuts, validation, liquid chromatography


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