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Title:Mikropropagacija plazeče zelene (Apium repens (Jacq.) Lag.) ekotip Kew, Velika Britanija
Authors:ID Bašič, Karmen (Author)
ID Ambrožič-Dolinšek, Jana (Mentor) More about this mentor... New window
Files:.pdf MAG_Basic_Karmen_2014.pdf (2,48 MB)
MD5: 516BDC61B706F47E709611F98B3F522B
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Namen naloge je bil vzpostaviti tkivno kulturo plazeče zelene (Apium repens (Jacq.) Lag.) iz semen, pridobljenih iz botaničnega vrta Kew v Veliki Britaniji, in vpeljati postopek hitrega razmnoževanja z mikropropagacijo. Za naše potrebe smo priredili protokol za mikropropagacijo španskega ekotipa plazeče zelene. Ekotip plazeče zelene iz Velike Britanije smo primerjali z ekotipom rastlin iz Španije. Na osnovno gojišče MS smo dodajali različne kombinacije in koncentracije rastnih regulatorjev BAP, 2iP, IBA in NAA. Rast in razvoj smo spremljali tako, da smo merili naslednje lastnosti: višina rastlin, število brstov, število stolonov, število novonastalih brstov na rastlinah in na stolonih, dolžina in število korenin. Plazeča zelena je najbolje uspevala na kontrolnem osnovnem gojišču MS brez rastnih regulatorjev. Največ brstov se je tvorilo na gojiščih MS s kombinacijo 2µM BAP in 0,5µM IBA ter s kombinacijo 2µM BAP in 0,5µM NAA. Korenine so se tvorile na vseh vcepkih na gojišču brez rastnih regulatorjev in z njimi. Zakoreninjene poganjke smo prestavili v zemljo, kjer se je uspešno aklimatiziralo vsaj 50 % rastlin.
Keywords:Apium repens, plazeča zelena, tkivna kultura, mikropropagacija, ekotip, Apiaceae, kobulnice, ex situ, ohranjanje, redke in ogrožene vrste
Place of publishing:Maribor
Publisher:[K. Bašič]
Year of publishing:2014
PID:20.500.12556/DKUM-46969 New window
UDC:581.16:582.795(41)(043.2)
COBISS.SI-ID:21019400 New window
NUK URN:URN:SI:UM:DK:KQLKC7EV
Publication date in DKUM:22.12.2014
Views:3119
Downloads:255
Metadata:XML DC-XML DC-RDF
Categories:FNM
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Secondary language

Language:English
Title:Micropropagation of Apium repens ecotype from Kew, United Kingdom
Abstract:The aim of the work was to establish a tissue culture and a micropropagation procedure of the creeping marshwort (Apium repens) obtained from The Royal Botanic Gardens Kew in United Kingdom. The objective of our study was to find out if the ecotype from Great Britain behaves the same as the ecotype from Spain. The tissue culture was established from seeds. We tested the effect of different growth regulators on the plant’s growth and development. Growth regulators that were added to the MS basic medium were: BAP, 2iP, IBA and NAA. We measured the plant’s height, the number of stolons, buds and roots and the length and the dispersion of roots. The majority of buds developed on the MS media by the use of the combination 2 µM BAP and 0.5µMIBA and of the combination 2 µM BAP and 0.5 µM NAA. The most successful plants grew on the MS medium without any added growth regulators. All plants developed roots no matter if there were any growth regulators used or not. Rooted plants were transferred to potting soil in which more than 50% of plants successfully survived acclimatization.
Keywords:Apium repens, tissue culture, micropropagation, ecotype, Apiaceae, ex situ, conservation, rare species


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