| Title: | Introduction and Optimization of the In-Vitro Method for Determining the Hemocompatibility of Modified Poly(Ethylene)Terephthalate Surfaces |
|---|
| Authors: | ID Stana, Jan (Author)
ID Stropnik, Domen (Author)
ID Strnad, Simona (Author)
ID Indest, Tea (Author)
ID Jevšek, Marko (Author)
ID Košir, Gorazd (Author) |
|---|
| Files: |  http://www.dlib.si/details/URN:NBN:SI:doc-AMLJAP7Y
 RAZ_Stana_Jan_2009.pdf (637,15 KB)
MD5: DCD6FF703E952D7C8D4EB4076ECC1C36
https://journals.um.si/index.php/amb/article/view/2057
|
|---|
| Language: | English |
|---|
| Work type: | Scientific work |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: | MF - Faculty of Medicine
|
|---|
| Abstract: | Purpose: Internationally-accepted standards have been developed for a range of tests and parameters for characterising the in-vitro interactions of biomaterials with blood. However, there are, as yet, no standards concerning the size, design and type of such in-vitro testing systems (1). Since the development of hemocompatible biomaterials provides a very important challenge in material science, there is a need for further progress in finding reliable and standardized methods for hemocompatibility testing (2). The aim of this research was to introduce a method for analyzing the hemocompatibility of different chemically-modified surfaces. Polyethylene terephthalate (PET), with surface modifications using different
polysaccharides and their derivatives, were chosen because of their promising biocompatible properties and numerous potential biomedical applications.
Methods: A modified hemoglobin-free method was used to determine the antithrombogenicity of the modified PET surfaces (4). The method was optimized for shaking rate, the addition of buffer, and blood temperature to decrease measuring errors. Five differently-modified PET surfaces were analyzed: chemically pre-treated PET and PET treated with chitosan, fucoidan, sulphated chitosan and heparin. Glass was used as a standard thrombogenic surface.
Results: The results showed that a lower shaking rate, the addition of buffer, and blood cooling prior to measurement significantly decreased the standard deviation of the measurement results by a total of about 89 %.
Conclusions: We believe this optimized hemoglobinfree method is suitable for distinguishing between chemically and structurally-different surfaces, such as glass and PET. The differences between PET surfaces coated with different polysaccharides were, however, less pronounced. |
|---|
| Keywords: | biomaterials, poly(ethyleneterephthalate), hemocompatibility, surface modification, in-vitro method |
|---|
| Place of publishing: | Maribor |
|---|
| Publisher: | Medicinska fakulteta |
|---|
| Year of publishing: | 2009 |
|---|
| Number of pages: | str. 61-67 |
|---|
| Numbering: | Letn. 2, št. 1 |
|---|
| PID: | 20.500.12556/DKUM-52576  |
|---|
| UDC: | 616 |
|---|
| ISSN on article: | 1855-5640 |
|---|
| COBISS.SI-ID: | 13224470 |
|---|
| DOI: | 10.18690/actabiomed.14 |
|---|
| NUK URN: | URN:SI:UM:DK:F9L8TNBC |
|---|
| Publication date in DKUM: | 10.07.2015 |
|---|
| Views: | 2354 |
|---|
| Downloads: | 71 |
|---|
| Metadata: |    |
|---|
| Categories: | Misc.
|
|---|
|
:
|
Copy citation |
|---|
| | | | Average score: | (0 votes) |
|---|
| Your score: | Voting is allowed only for logged in users. |
|---|
| Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
