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Title:BIOOGLJE KOT NOSILEC ZA IMOBILIZACIJO ENCIMA
Authors:ID Zadravec, Staša (Author)
ID Primožič, Mateja (Mentor) More about this mentor... New window
ID Leitgeb, Maja (Comentor)
ID Škerget, Mojca (Comentor)
Files:.pdf UN_Zadravec_Stasa_2015.pdf (1,68 MB)
MD5: 58BFE31FF18982C870752689EAE76DDC
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Namen diplomske naloge je bil uspešno imobilizirati encim na biooglje, nosilec, ki je bil pripravljen s hidrotermično karbonizacijo odpadne biomase. S spreminjanjem koncentracije encima in z različno izbiro mrežnega povezovalca smo želeli izvesti imobilizacijo encima na biooglje, ki nam da najvišjo preostalo aktivnost encima. Zanimalo nas je, kaj se z aktivnostjo imobiliziranega encima dogaja pri izpostavitvi različnim dejavnikom kot sta temperatura in čas skladiščenja. Rezultati kažejo, da najvišjo preostalo aktivnost obdrži imobiliziran encim s koncentracijo 0,05 g/mL ter ob uporabi mrežnega povezovalca gluteraldehida, koncentracije 2 %. Čas imobilizacije encima je bil 24 ur. Na stresalniku smo uporabili hitrost stresanja 400 rpm. Pri izpostavitvi imobiliziranega encima določeni temperaturi smo ugotovili, da dalj časa je izpostavljen višji temperaturi, bolj bo njegova aktivnost padala. Pri različnem času skladiščenja pa smo prišli do zaključka, da aktivnost encima s časom skladiščenja prav tako pada.
Keywords:biooglje, hidrotermična karbonizacija, imobilizacija encima, lakaza, gluteraldehid
Place of publishing:Maribor
Publisher:[S. Zadravec]
Year of publishing:2015
PID:20.500.12556/DKUM-54251 New window
UDC:66.046.562(043.2)
COBISS.SI-ID:19428118 New window
NUK URN:URN:SI:UM:DK:B34XRSGN
Publication date in DKUM:22.10.2015
Views:2054
Downloads:154
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Secondary language

Language:English
Title:BIOCHAR AS A CARRIER FOR ENZYME IMMOBILIZATION
Abstract:The purpose of the thesis was a successful immobilization of enzymes on biochar, a carrier, which was prepared from hydrothermal carbonization processes of biomass. By varying the concentration of the enzyme with a different choice of crosslinking agent, we want to perform immobilization of the enzyme on biochar, which gives the highest residual activity of the enzyme. We wanted to know, what is happening with the activity of the immobilized enzyme, when exposed to different conditions such as temperature and time of storage. The results show that the maximum residual activity of the immobilized enzyme was detected at enzyme concentration of 0.05 g/mL, using the crosslinking agent gluteraldehide, concentration of 2 %. The immobilization time of the enzyme was 24 hours. On a shaker, we used the agitation rate of 400 rpm. During the exposal of immobilized enzyme to a certain temperature, we found out that with the prolongation of exposition time of immobilized enzyme to the higher temperature, the activity of enzyme decreased. It was also detected that with the increase of storage time, the activity of immobilized enzyme decreased.
Keywords:biochar, hydrothermal carbonization, enzyme immobilization, laccase, gluteraldehid


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