| Title: | Effect of solid state fermentation medium optimization on Pleurotus ostreatus laccase production |
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| Authors: | ID Belšak Šel, Nataša (Author)
ID Gregori, Andrej (Author)
ID Leitgeb, Maja (Author)
ID Klinar, Dušan (Author)
ID Čelan, Štefan (Author) |
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| Files: |  Acta_Chimica_Slovenica_2015_Belsak-Sel_et_al._Effect_of_Solid_State_Fermentation_Medium_Optimization_on_Pleurotus_ostreatus_Laccase_Prod.pdf (126,99 KB)
MD5: E91624088702BD99AD48C9720ED17B7B
 https://journals.matheo.si/index.php/ACSi/article/view/1764
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | The objective of this work was to increase laccase production by Pleurotus ostreatus PLAB through culture medium optimization using solid-state culture conditions. Increased laccase activity was obtained through the design of experiments (DOE) using the Taguchi orthogonal array (OA). Seven factors, viz. lignocellulose, glucose, yeast extract, peptone, $KH_2PO_4$, $MgSO_4$ · $7H_2O$ and $MnSO_4$ · $H_2O$ at three levels and pH at two levels with OA layout of L18 ($2^1 × 3^7$) were selected for the proposed experimental design using Minitab 17 software. Data analysis showed that lignocellulose (20%) and glucose (10 g $L^{–1}$) had a positive effect, whereas $KH_2PO_4$, $MgSO_4$ · $7H_2O$ and $MnSO_4$ · $H_2O$ did not have a significant effect on laccase production. Taguchi OA analysis showed that pH 6, lignocellulose 20%, glucose 10 g $L^{–1}$, yeast extract 6 g $L^{–1}$, peptone 15 g $L^{–1}$, $KH_2PO_4$ 3 g $L^{–1}$, $MgSO_4$ · $7H_2O$ 0.5 g $L^{–1}$ and $MnSO_4$ · $H_2O$ 0.1 g $L^{–1}$ were the optimal conditions to maximize laccase production. The model predicted a 30.37 U $g^{–1}$ dry wt., which agreed with the experimentally obtained laccase activity 29.15 U $g^{–1}$ dry wt. at optimal conditions. |
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| Keywords: | Taguchi method (DOE), orthogonal array, optimization, Pleurotus ostreatus, laccase production, solid state fermentation |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2015 |
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| Number of pages: | str. 932-939 |
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| Numbering: | Letn. 62, št. 4 |
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| PID: | 20.500.12556/DKUM-60170  |
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| ISSN: | 1318-0207 |
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| UDC: | 60:577.152.1 |
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| ISSN on article: | 1318-0207 |
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| COBISS.SI-ID: | 19268374 |
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| DOI: | 10.17344/acsi.2015.1764 |
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| NUK URN: | URN:SI:UM:DK:S6UURWJK |
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| Publication date in DKUM: | 18.08.2017 |
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| Views: | 1889 |
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| Downloads: | 379 |
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| Metadata: |    |
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| Categories: | Misc.
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