| Title: | 16S rRNA in situ hybridization followed by flow cytometry for rapid identification of acetic acid bacteria involved in submerged industrial vinegar production |
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| Authors: | ID Trček, Janja (Author)
ID Lipoglavšek, Luka (Author)
ID Avguštin, Gorazd (Author) |
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| Files: |  Food_Technology_and_Biotechnology_2016_Trcek,_Lipoglavsek,_Avgustin_16S_rRNA_in_situ_Hybridization_Followed_by_Flow_Cytometry_for_Rapid.pdf (500,81 KB)
MD5: 7809705424A5B6A20D971CB67CD2D3E2
PID: 20.500.12556/dkum/7d57fe7f-ea23-45d0-9276-d7210ee370c2
 http://www.ftb.com.hr/index.php/archives/156-volume-54-issue-no-1/1404-16s-rrna-in-situ-hybridization-followed-by-flow-cytometry-for-rapid-identifi-cation-of-acetic-acid-bacteria-involved-in-submerged-industrial-vinegar-production
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FNM - Faculty of Natural Sciences and Mathematics
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| Abstract: | Acetic acid bacteria are involved in many biotechnological processes such as vitamin C, gluconic acid, miglitol or acetic acid production, and others. For a technologist trying to control the industrial process, the ability to follow the microbiological development of the process is thus of importance. During the past few years hybridization in a combination with flow cytometry has oft en been used for this purpose. Since vinegar is a liquid, it is an ideal matrix for flow cytometry analysis. In this work we have constructed a specific probe for highly acetic acid-resistant species of the acetic acid bacteria and a protocol for in situ hybridization, which in combination with flow cytometry enables direct monitoring of bacteria producing vinegar with >10 % of acetic acid. The approach was successfully applied for monitoring microbiota during industrial vinegar production. |
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| Keywords: | microbiology, acetic acid bacteria, flow cytometry |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 20.12.2014 |
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| Article acceptance date: | 13.10.2015 |
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| Publisher: | University of Zagreb Faculty of Food Technology and Biotechnology |
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| Year of publishing: | 2016 |
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| Number of pages: | 4 str.. |
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| Numbering: | Letn. 54, št. 1 |
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| PID: | 20.500.12556/DKUM-66997  |
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| ISSN: | 1330-9862 |
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| UDC: | 579.66 |
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| ISSN on article: | 1330-9862 |
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| COBISS.SI-ID: | 22612232 |
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| DOI: | 10.17113/ftb.54.01.16.4041 |
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| NUK URN: | URN:SI:UM:DK:QWDOJMCU |
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| Publication date in DKUM: | 24.07.2017 |
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| Views: | 27972 |
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| Downloads: | 426 |
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| Metadata: |    |
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| Categories: | Misc.
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