| Title: | Evaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure |
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| Authors: | ID Horvat, Sabina (Author)
ID Mahnič, Aleksander (Author)
ID Breskvar, Martin (Author)
ID Džeroski, Sašo (Author)
ID Rupnik, Maja (Author) |
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| Files: |  Scientific_Reports_2017_Horvat_et_al._Evaluating_the_effect_of_Clostridium_difficile_conditioned_medium_on_fecal_microbiota_community_st.pdf (1,82 MB)
MD5: D00136E00D21FAFC9BFE74827C11431F
PID: 20.500.12556/dkum/514a7e67-eb62-4806-b683-8ef6b943ae06
 http://www.nature.com/articles/s41598-017-15434-1
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
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| Abstract: | Clostridium difficile infection (CDI) is typically associated with disturbed gut microbiota and changes related to decreased colonization resistance against C. difficile are well described. However, nothing is known about possible effects of C. difficile on gut microbiota restoration during or after CDI. In this study, we have mimicked such a situation by using C. difficile conditioned medium of six different C. difficile strains belonging to PCR ribotypes 027 and 014/020 for cultivation of fecal microbiota. A marked decrease of microbial diversity was observed in conditioned medium of both tested ribotypes. The majority of differences occurred within the phylum Firmicutes, with a general decrease of gut commensals with putative protective functions (i.e. Lactobacillus, Clostridium_XIVa) and an increase in opportunistic pathogens (i.e. Enterococcus). Bacterial populations in conditioned medium differed between the two C. difficile ribotypes, 027 and 014/020 and are likely associated with nutrient availability. Fecal microbiota cultivated in medium conditioned by E. coli, Salmonella Enteritidis or Staphylococcus epidermidis grouped together and was clearly different from microbiota cultivated in C. difficile conditioned medium suggesting that C. difficile effects are specific. Our results show that the changes observed in microbiota of CDI patients are partially directly influenced by C. difficile. |
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| Keywords: | Clostridium difficile, infection, gut microbiota |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2017 |
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| Number of pages: | str. 1-11 |
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| Numbering: | Letn. 7, št. 1 |
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| PID: | 20.500.12556/DKUM-69227  |
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| ISSN: | 2045-2322 |
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| UDC: | 579.852.13 |
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| ISSN on article: | 2045-2322 |
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| COBISS.SI-ID: | 512751416 |
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| DOI: | 10.1038/s41598-017-15434-1 |
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| NUK URN: | URN:SI:UM:DK:3AKXLLXR |
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| Publication date in DKUM: | 12.12.2017 |
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| Views: | 1761 |
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| Downloads: | 521 |
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| Metadata: |    |
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| Categories: | Misc.
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