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Title:Strukturne značilnosti celic alfa Langerhansovih otočkov miši
Authors:ID Prosenica, Mia (Author)
ID Skelin Klemen, Maša (Mentor) More about this mentor... New window
ID Lipovšek, Saška (Comentor)
Files:.pdf MAG_Prosenica_Mia_2018.pdf (3,70 MB)
MD5: 332C222C43BCB1AE9FBEB21462D3DC0D
PID: 20.500.12556/dkum/a03292e0-0b70-4a1d-b1dd-0767b489a79e
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:V tej raziskavi je bil naš namen pregledati in primerjati ultrastrukturo celic alfa v Langerhansovih otočkih, v treh makroskopsko razdeljenih delih (spleničnem, gastričnem in duodenalnem delu) trebušne slinavke miši. Opazovali in primerjali smo velikosti glukagonskih veziklov in granul v tkivu, ki je bilo izpostavljeno različnim koncentracijam glukoze v različno dolgih časovnih intervalih. Vzorce tkiva smo analizirali s svetlobno mikroskopijo in presevno elektronsko mikroskopijo (TEM). Dobljene podatke smo statistično ovrednotili. Ugotovili smo, da je ultrastruktura organelov celic alfa v različnih delih trebušne slinavke in v tkivu, izpostavljenemu različnim koncentracijam glukoze, primerljiva. Statistično značilne razlike so bile opazne v premerih granul in veziklov ter v površini veziklov med tremi različnimi deli trebušne slinavke. Vzorci, izpostavljeni različnim koncentracijam glukoze v različno dolgih časovnih intervalih, so se statistično razlikovali v premerih granul in veziklov, v površini granul in veziklov ni bilo statističnih razlik. Med premeri in deleži površin granul in veziklov ob plazmalemi, ki so bili izpostavljeni različnim koncentracijam glukoze v različnem času, ni bilo statističnih razlik. Za nadaljnje raziskave bi bilo treba vključiti večje število Langerhansovih otočkov z vsebovanimi tipi celic, obravnavane tkivne rezine bi lahko bile izpostavljene še višjim koncentracijam glukoze in za daljše časovno obdobje.
Keywords:glukagon, glukoza, granule, miš, trebušna slinavka, ultrastruktura, vezikli
Place of publishing:Maribor
Publisher:[M. Prosenica]
Year of publishing:2018
PID:20.500.12556/DKUM-71919 New window
UDC:591.437:599.323.451(043.2)
COBISS.SI-ID:24118024 New window
NUK URN:URN:SI:UM:DK:JJOY4MGO
Publication date in DKUM:20.11.2018
Views:1849
Downloads:119
Metadata:XML DC-XML DC-RDF
Categories:FNM
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:04.09.2018

Secondary language

Language:English
Title:Structural characteristic in alpha cells of mouse langerhans islets
Abstract:In this study, our purpose was to examine and compare the ultrastructure of alpha cells in the islets of Langerhans in three macroscopically divided parts (splenetic, gastric and duodenal region) of the pancreas in mice. We observed and compared the size of glucagon vesicles and granules in the tissue, which was exposed to different glucose concentrations at various time intervals. With light microscopy and transmission electron microscopy (TEM) we analyzed tissue samples. The obtained data were statistically evaluated. We established that the ultrastructure of alpha cells organelles is similar in different parts of the pancreas and at various concentrations of glucose. Statistically significant differences were observed in the diameter of the granules and vesicles, and in the surface of the vesicles between the three different parts of the pancreas. Samples, exposed to different glucose concentrations at various time intervals, statistically differed in the diameter of the granules and vesicles, but no statistical differences were seen in the surface of granules and vesicles. Among the diameters and surface percentage of granules and vesicles near the cell membrane, which were exposed to different glucose concentrations at various time intervals, no statistical differences were seen. For further research, it would be necessary to include a larger number of islets with the contained cell types. Additionally, the treated tissue slices could be exposed to higher concentrations of glucose and for a significant period of time.
Keywords:glucagon, glucose, granules, mouse, pancreas, ultrastructure, vesicles


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